使用DNA探针检测伤寒热患者血液中的伤寒沙门氏菌。
Use of a DNA probe to detect Salmonella typhi in the blood of patients with typhoid fever.
作者信息
Rubin F A, McWhirter P D, Punjabi N H, Lane E, Sudarmono P, Pulungsih S P, Lesmana M, Kumala S, Kopecko D J, Hoffman S L
机构信息
Department of Bacterial Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
出版信息
J Clin Microbiol. 1989 May;27(5):1112-4. doi: 10.1128/jcm.27.5.1112-1114.1989.
A DNA probe was used to detect Salmonella typhi from blood samples from 14 of 33 patients with culture-confirmed typhoid fever, using the equivalent of 2.5 ml of blood. In contrast, S. typhi was detected in 17 of the same 33 patients by culture of 8 ml of blood. The probe hybridized to blood samples of 4 of 47 patients from whom S. typhi was not isolated.
使用DNA探针从33例血培养确诊为伤寒热的患者中的14例患者的血样中检测伤寒沙门氏菌，所用血量相当于2.5毫升。相比之下，通过对8毫升血液进行培养，在这33例相同患者中的17例检测到了伤寒沙门氏菌。该探针与47例未分离出伤寒沙门氏菌的患者的血样发生了杂交。
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