Vilar Marina Suênia de Araújo, de Souza Graziene Lopes, Vilar Daniela de Araújo, Leite Jacqueline Alves, Raffin Fernanda Nervo, Barbosa-Filho José Maria, Nogueira Fernando Henrique Andrade, Rodrigues-Mascarenhas Sandra, Moura Túlio Flávio Accioly de Lima
Biotechnology Center, Universidade Federal da Paraíba, João Pessoa-PB 58051-900, Brazil.
Department of Pharmacy, Federal University of Rio Grande do Norte, Natal-RN 59012-570, Brazil.
Molecules. 2016 Aug 19;21(8):1087. doi: 10.3390/molecules21081087.
The bark of A. occidentale L. is rich in tannins. Studies have described various biological activities of the plant, including antimicrobial, antioxidant, antiulcerogenic and antiinflammatory actions. The objective of this study was to assess the activity of the ethyl acetate phase (EtOAc) of A. occidentale on acute inflammation and to identify and quantify its phenolic compounds by HPLC. The method was validated and shown to be linear, precise and accurate for catechin, epicatechin, epigallocatechin and gallic acid. Swiss albino mice (Mus musculus) were treated with saline, Carrageenan (2.5%), Indomethacin (10 mg/kg), Bradykinin (6 nmol) and Prostaglandine E2 (5 µg) at different concentrations of EtOAc - A. occidentale (12.5; 25; 50; and 100 mg/kg/weight p.o.) for the paw edema test. Challenge was performed with carrageenan (500 µg/mL i.p.) for the doses 50 and 100 mg/kg of EtOAc. Levels of cytokines IL-1, TNF-α, IL-6 and IL-10 were also measured. All EtOAc - A. occidentale concentrations reduced the edema. At 50 and 100 mg/kg, an anti-inflammatory response of the EtOAc was observed. Carrageenan stimulus produced a neutrophil count of 28.6% while 50 and 100 mg/kg of the phase reduced this to 14.5% and 9.1%, respectively. The EtOAc extract reduced levels of IL-1 and TNF-α. These results suggest that the EtOAc plays a modulatory role in the inflammatory response. The chromatographic method can be used for the analysis of the phenolic compounds of the EtOAc phase.
西方滨藜（Atriplex occidentale L.）的树皮富含单宁。研究描述了该植物的多种生物活性，包括抗菌、抗氧化、抗溃疡和抗炎作用。本研究的目的是评估西方滨藜乙酸乙酯相（EtOAc）对急性炎症的活性，并通过高效液相色谱法（HPLC）鉴定和定量其酚类化合物。该方法经验证，对儿茶素、表儿茶素、表没食子儿茶素和没食子酸具有线性、精密度和准确度。在爪肿胀试验中，将瑞士白化小鼠（小家鼠）用生理盐水、角叉菜胶（2.5%）、吲哚美辛（10mg/kg）、缓激肽（6nmol）和前列腺素E2（5μg）以及不同浓度的西方滨藜乙酸乙酯相（12.5；25；50；和1百毫克/千克体重，口服）进行处理。对50和100mg/kg的乙酸乙酯相剂量，用角叉菜胶（500μg/mL，腹腔注射）进行激发。还测量了细胞因子白细胞介素-1（IL-1）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-10（IL-10）的水平。所有西方滨藜乙酸乙酯相浓度均降低了肿胀。在50和100mg/kg时，观察到乙酸乙酯相的抗炎反应。角叉菜胶刺激使中性粒细胞计数达到28.6%，而该相的50和100mg/kg剂量分别将其降至14.5%和9.1%。乙酸乙酯提取物降低了白细胞介素-1和肿瘤坏死因子-α的水平。这些结果表明，乙酸乙酯相在炎症反应中起调节作用。该色谱方法可用于分析乙酸乙酯相的酚类化合物。
