Department of Chemistry and Industrial Chemistry, University of Pisa, Via Moruzzi, 13, 56124, Pisa, Italy.
Top Curr Chem (Cham). 2016 Apr;374(2):20. doi: 10.1007/s41061-016-0020-8. Epub 2016 Mar 30.
The separation, detection and quantitation of specific species contained in a sample in the field of Cultural Heritage requires selective, sensitive and reliable methods. Procedures based on liquid chromatography fulfil these requirements and offer a wide range of applicability in terms of analyte types and concentration range. The main applications of High Performance Liquid Chromatography in this field are related to the separation and detection of dyestuffs in archaeological materials and paint samples by reversed-phase liquid chromatography with suitable detectors. The relevant literature will be revised, with particular attention to sample treatment strategies and future developments. Reversed phase chromatography has also recently gained increasing importance in the analysis of lipid binders and lipid materials in archaeological residues: the main advantages and disadvantages of the new approaches will be discussed. Finally, the main applications of ion chromatography and size exclusion chromatography in the field of Cultural Heritage will be revised in this chapter.
在文化遗产领域中，从样品中分离、检测和定量特定物种需要选择性、灵敏性和可靠性的方法。基于液相色谱的程序满足这些要求，并在分析物类型和浓度范围方面提供了广泛的适用性。高效液相色谱在该领域的主要应用涉及通过反相液相色谱法与合适的检测器分离和检测考古材料和油漆样品中的染料。将对相关文献进行综述，特别关注样品处理策略和未来发展。反相色谱法在分析考古残留物中的脂类结合剂和脂类材料方面也越来越重要：将讨论新方法的主要优缺点。最后，将在本章中综述离子色谱法和排阻色谱法在文化遗产领域的主要应用。
