Laboratory of Immunotherapy and Onco-Microbiome, University of Montreal Healthcare Research Center (CRCHUM), 900 Rue Saint-Denis, Montreal, QC, H2X 0A9, Canada.
Department of Pharmacology & Therapeutics, Faculty of Medicine and Health Sciences, McGill University, 3655 Promenade Sir-William-Osler, Montreal, QC, H3G 1Y6, Canada.
Arch Microbiol. 2023 Sep 24;205(10):338. doi: 10.1007/s00203-023-03671-0.
A polyphasic taxonomic approach, incorporating analysis of phenotypic features, cellular fatty acid profiles, 16S rRNA gene sequences, and determination of average nucleotide identity (ANI) plus digital DNA-DNA hybridization (dDDH), was applied to characterize an anaerobic bacterial strain designated KD22 isolated from human feces. 16S rRNA gene-based phylogenetic analysis showed that strain KD22 was found to be most closely related to species of the genus Gabonibacter. At the 16S rRNA gene level, the closest species from the strain KD22 corresponded with Gabonibacter massiliensis GM7, with a similarity of 97.58%. Cells of strain KD22 were Gram-negative coccobacillus, positive for indole and negative for catalase, nitrate reduction, oxidase, and urease activities. The fatty acid analysis demonstrated the presence of a high concentration of iso-C (51.65%). Next, the complete whole-genome sequence of strain KD22 was 3,368,578 bp long with 42 mol% of DNA G + C contents. The DDH and ANI values between KD22 and type strains of phylogenetically related species were 67.40% and 95.43%, respectively. These phylogenetic, phenotypic, and genomic results supported the affiliation of strain KD22 as a novel bacterial species within the genus Gabonibacter. The proposed name is Gabonibacter chumensis and the type strain is KD22 (= CSUR Q8104 = DSM 115208).
一种多相分类方法，结合表型特征分析、细胞脂肪酸图谱、16S rRNA 基因序列和平均核苷酸同一性（ANI）加上数字 DNA-DNA 杂交（dDDH）的测定，用于表征从人粪便中分离到的一株厌氧细菌菌株 KD22。基于 16S rRNA 基因的系统发育分析表明，菌株 KD22 与加蓬杆菌属的种最为密切相关。在 16S rRNA 基因水平上，与 KD22 菌株最接近的种是与加蓬杆菌属 GM7 相似性为 97.58%。KD22 菌株的细胞为革兰氏阴性球菌杆菌，吲哚阳性，过氧化氢酶、硝酸盐还原、氧化酶和脲酶活性阴性。脂肪酸分析表明存在高浓度的异-C（51.65%）。接下来，KD22 菌株的全基因组序列长 3368578bp，GC 含量为 42mol%。KD22 与系统发育相关种的模式株之间的 DDH 和 ANI 值分别为 67.40%和 95.43%。这些系统发育、表型和基因组结果支持 KD22 菌株属于加蓬杆菌属的一个新型细菌种。提议的名称是 Chumensis 加蓬杆菌，模式株是 KD22（= CSUR Q8104 = DSM 115208）。
