Mozhdeganloo Z, Moghadam Jafari A, Koohi M K, Heidarpour M
Post-Graduate Student, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;
Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran;
Iran J Vet Res. 2016 Winter;17(1):31-5.
The objective of this study was to investigate the propensity of permethrin (PTN) to induce oxidative stress and changes in enzyme activities in liver of rainbow trout and its possible attenuation by vitamin C. Forty-eight fish were randomly assigned to 1 of 6 treatment groups and their livers were used for liver perfusion method: control (0 µgL(-1) permethrin and 0 mgL(-1) vitamin C), PTN-0.16 (0.16 µgL(-1) permethrin), PTN-0.32 (0.32 µgL(-1) permethrin), PTN-0.64 (0.64 µgL(-1) permethrin), Vit. C (17.2 mgL(-1) vitamin C), and PTN-0.64 + Vit. C (0.64 µgL(-1) permethrin and 17.2 mgL(-1) vitamin C). Results obtained showed that permethrin significantly (P<0.05) increased ALT, AST and LDH activities in the liver perfusion medium and malondialdehyde (MDA) level in liver tissue. The values of reduced glutathione (GSH) and total antioxidant capacity (FRAP) in the liver tissue were significantly decreased due to permethrin administration. Pearson's correlation analysis revealed a positive correlation between MDA concentration and ALT, AST and LDH activities in the permethrin groups, suggesting that the enhanced lipid peroxidation may be linked to hepatic damage caused by permethrin. On the other hand, treatment with vitamin C in the PTN-0.64 + Vit. C group increased the values of GSH and FRAP, and decreased the level of MDA and the activities of hepatic enzymes, when compared to the PTN-0.64 group. The present study revealed that vitamin C could ameliorate permethrin-induced oxidative damage by decreasing lipid peroxidation and altering antioxidant defense system in liver of rainbow trout.
本研究的目的是调查氯菊酯（PTN）诱导虹鳟鱼肝脏氧化应激和酶活性变化的倾向以及维生素C对其可能的缓解作用。48条鱼被随机分配到6个处理组中的1组，并使用其肝脏进行肝脏灌注实验：对照组（0 µgL(-1)氯菊酯和0 mgL(-1)维生素C）、PTN-0.16组（0.16 µgL(-1)氯菊酯）、PTN-0.32组（0.32 µgL(-1)氯菊酯）、PTN-0.64组（0.64 µgL(-1)氯菊酯）、维生素C组（17.2 mgL(-1)维生素C）以及PTN-0.64 +维生素C组（0.64 µgL(-1)氯菊酯和17.2 mgL(-1)维生素C）。所得结果表明，氯菊酯显著（P<0.05）增加了肝脏灌注培养基中的谷丙转氨酶（ALT）、谷草转氨酶（AST）和乳酸脱氢酶（LDH）活性以及肝脏组织中的丙二醛（MDA）水平。由于给予氯菊酯，肝脏组织中还原型谷胱甘肽（GSH）和总抗氧化能力（FRAP）的值显著降低。Pearson相关性分析显示，氯菊酯组中MDA浓度与ALT、AST和LDH活性之间呈正相关，表明脂质过氧化增强可能与氯菊酯引起的肝脏损伤有关。另一方面，与PTN-0.64组相比，PTN-0.64 +维生素C组用维生素C处理后，GSH和FRAP的值增加，MDA水平和肝脏酶活性降低。本研究表明，维生素C可通过减少脂质过氧化和改变虹鳟鱼肝脏中的抗氧化防御系统来减轻氯菊酯诱导的氧化损伤。
