库蚊黄病毒（CxFV）的基因组测序和遗传特征分析提供了有关其基因型的新信息。
Genome sequencing and genetic characterization of Culex Flavirirus (CxFV) provides new information about its genotypes.
作者信息
Bittar Cíntia, Machado Daiane Cristina, Vedovello Danila, Ullmann Leila Sabrina, Rahal Paula, Araújo Junior João Pessoa, Nogueira Maurício Lacerda
机构信息
UNESP - São Paulo State University, Rua Cristóvão Colombo, 2265 - Jardim Nazareth, CEP 15054-000, São José do Rio Preto, SP, Brazil.
FAMERP - Faculdade de Medicina de São José do Rio Preto, Av. Brigadeiro Faria Lima, 5416, Bloco U6 - Vila São Pedro, CEP: 15090-000, São José do Rio Preto, SP, Brazil.
出版信息
Virol J. 2016 Sep 23;13(1):158. doi: 10.1186/s12985-016-0614-3.
BACKGROUND
Culex Flavivirus (CxFV) is an insect-specific virus that is widely distributed and primarily infects mosquito species from the genus Culex. Its hosts include Culex tritaeniorhynchus, Culex quinquefasciatus, and Anopheles sinensis mosquitoes. Since its original identification, CxFV has been reported in several countries. Despite the increasing number of reports on CxFV, little is known about its genomic characteristics. It is unclear whether the phylogenetic relationships between the strains are influenced by host species and geographic location.
RESULTS
We characterized the Brazilian CxFV strain and performed a comprehensive genetic and phylogenetic characterization of CxFV based on all ORF sequences described so far. Our results revealed that the Brazilian strain is in a monophyletic clade with the Mexican strain. Overall, selective pressure indicates that the ORF is undergoing purifying selection.
CONCLUSIONS
The phylogenetic analysis revealed a strong association between climate and CxFV ancestry. Also, based on phylogeny and the genetic distance between the main branches of the tree, we propose the classification of the available sequences into two different genotypes. We also suggest the existence of two different subtypes within Genotype 1.
背景
库蚊黄病毒（CxFV）是一种昆虫特异性病毒，广泛分布，主要感染库蚊属的蚊虫种类。其宿主包括三带喙库蚊、致倦库蚊和中华按蚊。自最初被鉴定以来，多个国家都报告发现了CxFV。尽管关于CxFV的报告数量不断增加，但其基因组特征却鲜为人知。目前尚不清楚病毒株之间的系统发育关系是否受宿主种类和地理位置的影响。
结果
我们对巴西CxFV毒株进行了特征分析，并基于目前已描述的所有开放阅读框（ORF）序列对CxFV进行了全面的遗传和系统发育特征分析。我们的结果显示，巴西毒株与墨西哥毒株处于一个单系分支中。总体而言，选择压力表明该ORF正在经历纯化选择。
结论
系统发育分析揭示了气候与CxFV谱系之间的紧密关联。此外，基于系统发育和树的主要分支之间的遗传距离，我们建议将现有序列分为两种不同的基因型。我们还认为在基因型1中存在两种不同的亚型。
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