Shadowen R D, Sciortino C V
Department of Medicine, Medical University of South Carolina, Charleston 29425.
J Clin Microbiol. 1989 Aug;27(8):1744-7. doi: 10.1128/jcm.27.8.1744-1747.1989.
The recovery of Campylobacter pylori from clinical specimens is difficult, even when done with an optimal medium, atmosphere, and temperature. The growth of this organism was investigated by comparing a biphasic system with broth culture. The effects of gyration, inoculum, and pH were studied. Brucella agar and broth supplemented with 2.5% fetal bovine serum were used. Growth in the biphasic system was an average of 2 log units (7 X 10(8) versus 5 X 10(6) CFU/ml) greater than that in the broth system (P less than 0.01), and this occurred 12 to 24 h sooner in the biphasic system. When gyration was added, an average of 1 log unit of growth improvement was seen in comparable systems. Improved growth was also seen with low inoculum levels, in which stationary-phase cells in the broth system reached 10(5) CFU/ml compared with 10(7) CFU/ml in the biphasic system. At the three pH ranges studied, growth was best at pH 8 to 9 (6 X 10(9) CFU/ml), averaging 2 log units greater growth than that at pH 6 to 7 and 4 log units greater growth than that at pH 4.5 to 5.5 (P less than 0.01). The improved recovery of the organism for low inoculum levels in a biphasic system may be important for long-term storage and clinical isolation.
即使在使用最佳培养基、培养环境和温度的情况下，从临床标本中培养出幽门螺杆菌也很困难。通过比较双相系统和肉汤培养法，对该菌的生长情况进行了研究。研究了旋转、接种量和pH值的影响。使用了补充有2.5%胎牛血清的布鲁氏琼脂和肉汤。双相系统中的生长量平均比肉汤系统高2个对数单位（7×10⁸对5×10⁶CFU/ml）（P<0.01），且双相系统中生长提前12至24小时出现。当添加旋转时，在可比系统中平均生长改善1个对数单位。在低接种量水平下也观察到生长改善，肉汤系统中的稳定期细胞达到10⁵CFU/ml，而双相系统中为10⁷CFU/ml。在所研究的三个pH范围内，pH 8至9时生长最佳（6×10⁹CFU/ml），平均生长量比pH 6至7时高2个对数单位，比pH 4.5至5.5时高4个对数单位（P<0.01）。双相系统中低接种量水平下该菌回收率提高，这对于长期保存和临床分离可能很重要。
