Saluja Tarun, Dhingra Mandeep S, Sharma Shiv D, Gupta Madhu, Kundu Ritabrata, Kar Sonali, Dutta Ashok K, Silveira Maria D P, Singh Jai V, Kamath Veena G, Chaudhary Anurag, Rao Venkateswara, Ravi Mandyam D, Murthy Kesava, Arumugam Rajesh, Moureau Annick, Prasad Rajendra, Patnaik Badri N
a Shantha Biotechnics Pvt. Ltd. , Hyderabad , India.
b Sanofi Pasteur , Swiftwater , PA, USA.
Hum Vaccin Immunother. 2017 Mar 4;13(3):711-716. doi: 10.1080/21645515.2016.1238994. Epub 2016 Sep 29.
Rotavirus is the leading cause of severe and dehydrating diarrhea in children aged under 5 years. We undertook this hospital-based surveillance study to examine the possible relationship between the severity of diarrhea and the various G-group rotaviruses circulating in India. Stool samples (n = 2,051) were systematically collected from 4,711 children aged <5 years admitted with severe acute gastroenteritis to 12 medical school centers from April 2011 to July 2012. Rotavirus testing was undertaken using a commercially available enzyme immunoassay kit for the rotavirus VP6 antigen (Premier Rotaclone Qualitative ELISA). Rotavirus positive samples were genotyped for VP7 and VP4 antigens by reverse-transcription polymerase chain reaction at a central laboratory. Of the stool samples tested for rotavirus antigen, 541 (26.4%) were positive for VP6 antigen. Single serotype infections from 377 stool samples were compared in terms of gastroenteritis severity. Among those with G1 rotavirus infection, very severe diarrhea (Vesikari score ≥ 16) was reported in 59 (33.9%) children, severe diarrhea (Vesikari score 11-15) in 104 (59.8%), moderate (Vesikari score 6-10) and mild diarrhea (Vesikari score 0-5) in 11 (6.3%). Among those with G2 infection, very severe diarrhea was reported in 26 (27.4%) children, severe diarrhea in 46 (48.4%), and moderate and mild diarrhea in 23 (24.2 %). Among those with G9 infection, very severe diarrhea was reported in 47 (54.5%) children, severe diarrhea in 29 (33.6%), and moderate and mild diarrhea in 10 (11.9%). Among those with G12 infection, very severe diarrhea was reported in 9 (40.9%) children and severe diarrhea in 13 (59.1%). The results of this study indicate some association between rotavirus serotypes and severity of gastroenteritis.
轮状病毒是5岁以下儿童严重腹泻和脱水腹泻的主要病因。我们开展了这项基于医院的监测研究，以探讨腹泻严重程度与印度流行的各种G组轮状病毒之间的可能关系。2011年4月至2012年7月期间，从12所医学院中心收治的4711名5岁以下严重急性胃肠炎患儿中系统收集了粪便样本（n = 2051）。使用市售的轮状病毒VP6抗原酶免疫分析试剂盒（Premier Rotaclone定性ELISA）进行轮状病毒检测。轮状病毒阳性样本在中央实验室通过逆转录聚合酶链反应对VP7和VP4抗原进行基因分型。在检测轮状病毒抗原的粪便样本中，541份（26.4%）VP6抗原呈阳性。对377份粪便样本的单血清型感染情况就胃肠炎严重程度进行了比较。在感染G1轮状病毒的患儿中，59名（33.9%）出现极重度腹泻（维西卡里评分≥16），104名（59.8%）出现重度腹泻（维西卡里评分11 - 15），11名（6.3%）出现中度（维西卡里评分6 - 10）和轻度腹泻（维西卡里评分0 - 5）。在感染G2的患儿中，26名（27.4%）出现极重度腹泻，46名（48.4%）出现重度腹泻，23名（24.2%）出现中度和轻度腹泻。在感染G9的患儿中，47名（54.5%）出现极重度腹泻，29名（33.6%）出现重度腹泻，10名（11.9%）出现中度和轻度腹泻。在感染G12的患儿中，9名（40.9%）出现极重度腹泻，13名（59.1%）出现重度腹泻。本研究结果表明轮状病毒血清型与胃肠炎严重程度之间存在一定关联。
