Sharma Hunny, Yunus G Y, Agrawal Rohit, Kalra Monika, Verma Swati, Bhattar Supriya
Department of Public Health Dentistry, Rungta College of Dental Science and Research, Bhilai, Chhattisgarh, India.
Department of Public Health Dentistry, Teerthanker Mahaveer Dental College and Research Centre, Moradabad, Uttar Pradesh, India.
Indian J Dent Res. 2016 Jul-Aug;27(4):433-436. doi: 10.4103/0970-9290.191895.
From ancient times, plants with medicinal values are being tested and used in the treatment of various infectious diseases.
The present in vitro study was designed to assess the antifungal activity of three commonly available medicinal plants Glycyrrhiza glabra, Ficus religiosa, and Plantago major on inhibiting oral Candida albicans in comparison to standard antifungal agents.
Bark of G. glabra, stem of F. religiosa, and husk of P. major were collected, crushed into fine powder, and dissolved in 67% ethanol. Extracts were subjected to antifungal efficacy test against oral C. albicans (ATCC 66027) using Kirby-Bauer disc diffusion method. Mean zone of inhibition (ZOI) was measured by HI antibiotic zone scale. One-way ANOVA using Tukey's post hoc and t-test were applied for statistical analysis.
G. glabra was found to be most effective among the three with highest mean ZOI measuring 19.8 ± 0.83, 19.4 ± 0.54, and 18.2 ± 1.09 at 24, 48, and 72 h, respectively. Tukey's post hoc test showed statistically nonsignificant difference between antifungal activity of F. religiosa and P. major with itraconazole 10 mcg.
G. glabra, F. religiosa, and P. major showed acceptable potency against C. albicans (ATCC 66027) comparable to that of synthetic antifungal agents. However, further studies should be undertaken to affirm the same and test their efficacy in different concentrations and clinical utility.
自古以来，具有药用价值的植物就被用于测试和治疗各种传染病。
本体外研究旨在评估三种常见药用植物光果甘草、菩提树和大车前草对抑制口腔白色念珠菌的抗真菌活性，并与标准抗真菌药物进行比较。
收集光果甘草的树皮、菩提树的茎和大车前草的外壳，粉碎成细粉，溶于67%乙醇中。采用 Kirby-Bauer 纸片扩散法对提取物进行抗口腔白色念珠菌（ATCC 66027）的抗真菌疗效测试。使用 HI 抗生素抑菌圈测量仪测量平均抑菌圈（ZOI）。采用 Tukey 事后检验的单因素方差分析和 t 检验进行统计分析。
在这三种植物中，光果甘草最为有效，在24、48和72小时时的平均抑菌圈最高，分别为19.8±0.83、19.4±0.54和18.2±1.09。Tukey 事后检验显示，菩提树和大车前草与10 mcg 伊曲康唑的抗真菌活性之间在统计学上无显著差异。
光果甘草、菩提树和大车前草对白色念珠菌（ATCC 66027）显示出与合成抗真菌药物相当的可接受效力。然而，应进行进一步研究以证实这一点，并测试它们在不同浓度下的疗效和临床实用性。
