Niu Jun, Wang Jia, An Jiyong, Liu Lili, Lin Zixin, Wang Rui, Wang Libing, Ma Chao, Shi Lingling, Lin Shanzhi
College of Biological Sciences and Biotechnology, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.
Research Institute of Forestry, Chinese Academy of Forestry, Beijing 10091, China.
Sci Rep. 2016 Oct 20;6:35675. doi: 10.1038/srep35675.
Recently, our transcriptomic analysis has identified some functional genes responsible for oil biosynthesis in developing SASK, yet miRNA-mediated regulation for SASK development and oil accumulation is poorly understood. Here, 3 representative periods of 10, 30 and 60 DAF were selected for sRNA sequencing based on the dynamic patterns of growth tendency and oil content of developing SASK. By miRNA transcriptomic analysis, we characterized 296 known and 44 novel miRNAs in developing SASK, among which 36 known and 6 novel miRNAs respond specifically to developing SASK. Importantly, we performed an integrated analysis of mRNA and miRNA transcriptome as well as qRT-PCR detection to identify some key miRNAs and their targets (miR156-SPL, miR160-ARF18, miR164-NAC1, miR171h-SCL6, miR172-AP2, miR395-AUX22B, miR530-P2C37, miR393h-TIR1/AFB2 and psi-miRn5-SnRK2A) potentially involved in developing response and hormone signaling of SASK. Our results provide new insights into the important regulatory function of cross-talk between development response and hormone signaling for SASK oil accumulation.
最近，我们的转录组分析已经鉴定出一些在发育中的紫苏种子中负责油脂生物合成的功能基因，但对于紫苏种子发育和油脂积累的miRNA介导的调控了解甚少。在此，根据发育中的紫苏种子的生长趋势和油脂含量的动态模式，选择了开花后10天、30天和60天这3个代表性时期进行小RNA测序。通过miRNA转录组分析，我们在发育中的紫苏种子中鉴定出296个已知的和44个新的miRNA，其中36个已知的和6个新的miRNA对发育中的紫苏种子有特异性反应。重要的是，我们对mRNA和miRNA转录组进行了综合分析以及qRT-PCR检测，以鉴定一些关键的miRNA及其靶标（miR156-SPL、miR160-ARF18、miR164-NAC1、miR171h-SCL6、miR172-AP2、miR395-AUX22B、miR530-P2C37、miR393h-TIR1/AFB2和psi-miRn5-SnRK2A），它们可能参与紫苏种子的发育反应和激素信号传导。我们的结果为发育反应和激素信号传导之间的相互作用对紫苏种子油脂积累的重要调控功能提供了新的见解。
