用于定量真菌的新型荧光检测法。
New fluorescence assay for the quantitation of fungi.
作者信息
Coleman T, Madassery J V, Kobayashi G S, Nahm M H, Little J R
机构信息
Infectious Diseases Division, Jewish Hospital, St. Louis, Missouri.
出版信息
J Clin Microbiol. 1989 Sep;27(9):2003-7. doi: 10.1128/jcm.27.9.2003-2007.1989.
Abstract
Quantitative determination of fungal mass is easily achieved with a new procedure that detects particle epifluorescence. Fungi are detected after exposure to a fluorescent stain (Fungiqual; CIBA-GEIGY Corp., Summit, N.J.) by using a fluorescence particle concentration analyzer. This report describes a simple fluorescence method for quantitation of either yeast or mycelial forms of fungi. The nature of the staining reaction was studied, and a practical application of this procedure for determination of fungal susceptibility to an antifungal agent is presented.
摘要
采用一种检测颗粒落射荧光的新方法可轻松实现真菌量的定量测定。通过使用荧光颗粒浓度分析仪，在真菌暴露于荧光染料（Fungiqual；CIBA - GEIGY公司，新泽西州萨米特）后对其进行检测。本报告描述了一种用于定量酵母菌或丝状真菌形式的简单荧光方法。研究了染色反应的性质，并介绍了该方法在测定真菌对抗真菌剂敏感性方面的实际应用。
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