• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
New fluorescence assay for the quantitation of fungi.用于定量真菌的新型荧光检测法。
J Clin Microbiol. 1989 Sep;27(9):2003-7. doi: 10.1128/jcm.27.9.2003-2007.1989.
2
CTBT (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine) producing ROS affects growth and viability of filamentous fungi.CTBT（7-氯四唑并[5,1-c]苯并[1,2,4]三嗪）产生 ROS 影响丝状真菌的生长和活力。
FEMS Microbiol Lett. 2012 Mar;328(2):138-43. doi: 10.1111/j.1574-6968.2011.02491.x. Epub 2012 Jan 10.
3
In vitro activity of aureofungin, amphotericin B and hamycin on the mycelial growth of some thermophilic fungi.
Hindustan Antibiot Bull. 1973 Feb;15(3):75-8.
4
Comparative study of amphotericin-B pimaricin and gentian violet on ocular fungi.两性霉素B、匹马霉素和龙胆紫对眼部真菌的对比研究。
Indian J Ophthalmol. 1986;34:73-7.
5
Rapid viability assessment of yeast cells using vital staining with 2-NBDG, a fluorescent derivative of glucose.使用2-NBDG（葡萄糖的荧光衍生物）进行活细胞染色对酵母细胞进行快速活力评估。
Int J Food Microbiol. 2002 Jun 5;76(1-2):47-53. doi: 10.1016/s0168-1605(01)00750-4.
6
A chemically modified tetracycline (CMT-3) is a new antifungal agent.一种化学修饰的四环素（CMT-3）是一种新型抗真菌剂。
Antimicrob Agents Chemother. 2002 May;46(5):1447-54. doi: 10.1128/AAC.46.5.1447-1454.2002.
7
Comparative in vitro antifungal activity of amphotericin B and amphotericin B methyl ester.两性霉素B与两性霉素B甲酯的体外抗真菌活性比较
Antimicrob Agents Chemother. 1975 Jan;7(1):58-63. doi: 10.1128/AAC.7.1.58.
8
In vitro efficacy of antifungal treatment using riboflavin/UV-A (365 nm) combination and amphotericin B.使用核黄素/UV-A（365nm）联合治疗和两性霉素 B 的体外疗效。
Invest Ophthalmol Vis Sci. 2010 Aug;51(8):3950-3. doi: 10.1167/iovs.09-4013. Epub 2010 Mar 24.
9
Use of an antifungal drug, amphotericin B for isolation of thraustochytrids.使用抗真菌药物两性霉素 B 分离甲藻。
J Biosci Bioeng. 2010 Dec;110(6):720-3. doi: 10.1016/j.jbiosc.2010.07.011. Epub 2010 Aug 12.
10
Antifungal drug susceptibility of oral Candida albicans isolates may be associated with apoptotic responses to Amphotericin B.口腔白色念珠菌分离株的抗真菌药物敏感性可能与两性霉素 B 诱导的细胞凋亡反应有关。
J Oral Pathol Med. 2010 Feb;39(2):182-7. doi: 10.1111/j.1600-0714.2009.00811.x. Epub 2009 Jul 27.

引用本文的文献

1
Cell-wall dyes interfere with Cryptococcus neoformans melanin deposition.细胞壁染料会干扰新型隐球菌黑色素的沉积。
Microbiology (Reading). 2018 Aug;164(8):1012-1022. doi: 10.1099/mic.0.000682. Epub 2018 Jun 25.
2
N-acetylglucosamine affects Cryptococcus neoformans cell-wall composition and melanin architecture.N-乙酰葡萄糖胺影响新型隐球菌细胞壁组成和黑色素结构。
Microbiology (Reading). 2017 Nov;163(11):1540-1556. doi: 10.1099/mic.0.000552. Epub 2017 Oct 18.
3
Characterization of the antifungal functions of a WGA-Fc (IgG2a) fusion protein binding to cell wall chitin oligomers.鉴定与细胞壁几丁寡糖结合的 WGA-Fc（IgG2a）融合蛋白的抗真菌功能。
Sci Rep. 2017 Sep 22;7(1):12187. doi: 10.1038/s41598-017-12540-y.
4
Rapid protocol for visualization of rust fungi structures using fluorochrome Uvitex 2B.使用荧光增白剂Uvitex 2B可视化锈菌结构的快速方案。
Plant Methods. 2015 Dec 11;11:54. doi: 10.1186/s13007-015-0096-0. eCollection 2015.
5
Solvatochromic effect in the optical spectra of calcofluor and its relation to fluorescent staining of yeast cell walls.溶剂变色效应在Calcofluor 光学光谱中的表现及其与酵母细胞壁荧光染色的关系。
J Fluoresc. 2010 Jan;20(1):343-52. doi: 10.1007/s10895-009-0563-y. Epub 2009 Oct 31.
6
Diagnosis of invasive mycoses in severely immunosuppressed patients.严重免疫抑制患者侵袭性真菌病的诊断
Ann Hematol. 1993 Jul;67(1):1-11. doi: 10.1007/BF01709659.
7
Antifungal susceptibility testing.抗真菌药敏试验
Clin Microbiol Rev. 1993 Oct;6(4):367-81. doi: 10.1128/CMR.6.4.367.
8
Spectrophotometric method of inoculum preparation for the in vitro susceptibility testing of filamentous fungi.丝状真菌体外药敏试验接种体制备的分光光度法
J Clin Microbiol. 1991 Feb;29(2):393-4. doi: 10.1128/jcm.29.2.393-394.1991.
9
Comparison study of broth macrodilution and microdilution antifungal susceptibility tests.肉汤稀释法和微量稀释法抗真菌药敏试验的比较研究
J Clin Microbiol. 1991 Jun;29(6):1089-94. doi: 10.1128/jcm.29.6.1089-1094.1991.
10
Collaborative comparison of broth macrodilution and microdilution antifungal susceptibility tests.肉汤稀释法和微量稀释法抗真菌药敏试验的协同比较
J Clin Microbiol. 1992 Dec;30(12):3138-45. doi: 10.1128/jcm.30.12.3138-3145.1992.

本文引用的文献

1
Nonencapsulated Variant of Cryptococcus neoformans I. Virulence Studies and Characterization of Soluble Polysaccharide.新型隐球菌非包被变异株 I. 毒力研究及可溶性多糖的特性。
Infect Immun. 1971 Feb;3(2):287-94. doi: 10.1128/iai.3.2.287-294.1971.
2
Cysteine-independent and cysteine-requiring yeast-strains of Histoplasma capsulatum.荚膜组织胞浆菌的不依赖半胱氨酸和需要半胱氨酸的酵母菌株。
Mycopathologia. 1982 Feb 19;77(2):69-73. doi: 10.1007/BF00437386.
3
Microscopic studies of Candida albicans and Torulopsis glabrata after in vitro treatment with bifonazole. Light and scanning electron microscopy.用联苯苄唑体外处理白色念珠菌和光滑球拟酵母后的显微镜研究。光学显微镜和扫描电子显微镜观察。
Arzneimittelforschung. 1983;33(4):528-37.
4
Specificity of binding of hexopyranosyl polysaccharides with fluorescent brightener.己吡喃糖基多糖与荧光增白剂的结合特异性
J Biochem. 1967 Aug;62(2):276-8. doi: 10.1093/oxfordjournals.jbchem.a128660.
5
Isolation and characterization of spontaneous avirulent variants of Histoplasma capsulatum.
Infect Immun. 1987 Mar;55(3):528-33. doi: 10.1128/iai.55.3.528-533.1987.
6
A rapid fluorescent assay to distinguish attached from phagocytized yeast particles.一种用于区分附着的酵母颗粒和被吞噬的酵母颗粒的快速荧光检测法。
J Immunol Methods. 1987 Jul 16;101(1):37-42. doi: 10.1016/0022-1759(87)90213-4.
7
Encapsulation and melanin formation as indicators of virulence in Cryptococcus neoformans.荚膜形成和黑色素生成作为新型隐球菌毒力的指标
Infect Immun. 1986 Jan;51(1):218-23. doi: 10.1128/iai.51.1.218-223.1986.
8
A comparison of the highly selective fluorescence staining of fungi in tissue sections with Uvitex 2B and Calcofluor White M2R.使用Uvitex 2B和荧光增白剂M2R对组织切片中的真菌进行高选择性荧光染色的比较。
Histochem J. 1988 Apr;20(4):215-21. doi: 10.1007/BF01747466.
9
Modulation of the macrophage oxidative burst by Histoplasma capsulatum.荚膜组织胞浆菌对巨噬细胞氧化爆发的调节作用。
J Immunol. 1987 Jan 15;138(2):582-6.
10
Timing and function of chitin synthesis in yeast.酵母中几丁质合成的时间和功能。
J Bacteriol. 1975 Dec;124(3):1586-93. doi: 10.1128/jb.124.3.1586-1593.1975.

用于定量真菌的新型荧光检测法。

New fluorescence assay for the quantitation of fungi.

作者信息

Coleman T, Madassery J V, Kobayashi G S, Nahm M H, Little J R

机构信息

Infectious Diseases Division, Jewish Hospital, St. Louis, Missouri.

出版信息

J Clin Microbiol. 1989 Sep;27(9):2003-7. doi: 10.1128/jcm.27.9.2003-2007.1989.

DOI:10.1128/jcm.27.9.2003-2007.1989
PMID:2778063
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267727/
Abstract

Quantitative determination of fungal mass is easily achieved with a new procedure that detects particle epifluorescence. Fungi are detected after exposure to a fluorescent stain (Fungiqual; CIBA-GEIGY Corp., Summit, N.J.) by using a fluorescence particle concentration analyzer. This report describes a simple fluorescence method for quantitation of either yeast or mycelial forms of fungi. The nature of the staining reaction was studied, and a practical application of this procedure for determination of fungal susceptibility to an antifungal agent is presented.

摘要

采用一种检测颗粒落射荧光的新方法可轻松实现真菌量的定量测定。通过使用荧光颗粒浓度分析仪，在真菌暴露于荧光染料（Fungiqual；CIBA - GEIGY公司，新泽西州萨米特）后对其进行检测。本报告描述了一种用于定量酵母菌或丝状真菌形式的简单荧光方法。研究了染色反应的性质，并介绍了该方法在测定真菌对抗真菌剂敏感性方面的实际应用。