Guisbiers Grégory, Lara Humberto H, Mendoza-Cruz Ruben, Naranjo Guillermo, Vincent Brandy A, Peralta Xomalin G, Nash Kelly L
The University of Texas at San Antonio, Department of Physics and Astronomy, One UTSA Circle, San Antonio, TX, USA.
The University of Texas at San Antonio, Department of Physics and Astronomy, One UTSA Circle, San Antonio, TX, USA.
Nanomedicine. 2017 Apr;13(3):1095-1103. doi: 10.1016/j.nano.2016.10.011. Epub 2016 Oct 25.
Selenoproteins play an important role in the human body by accomplishing essential biological functions like oxido-reductions, antioxidant defense, thyroid hormone metabolism and immune response; therefore, the possibility to synthesize selenium nanoparticles free of any contaminants is exciting for future nano-medical applications. This paper reports the first synthesis of selenium nanoparticles by femtosecond pulsed laser ablation in de-ionized water. Those pure nanoparticles have been successfully used to inhibit the formation of Candida albicans biofilms. Advanced electron microscopy images showed that selenium nanoparticles easily adhere on the biofilm, then penetrate into the pathogen, and consequently damage the cell structure by substituting with sulfur. 50% inhibition of Candida albicans biofilm was obtained at only 25 ppm. Finally, the two physical parameters proved to affect strongly the viability of Candida albicans are the crystallinity and particle size.
硒蛋白通过完成氧化还原、抗氧化防御、甲状腺激素代谢和免疫反应等重要生物学功能，在人体中发挥着重要作用；因此，合成不含任何污染物的硒纳米颗粒的可能性对于未来的纳米医学应用来说令人兴奋。本文报道了首次在去离子水中通过飞秒脉冲激光烧蚀合成硒纳米颗粒。那些纯纳米颗粒已成功用于抑制白色念珠菌生物膜的形成。先进的电子显微镜图像显示，硒纳米颗粒很容易附着在生物膜上，然后渗透到病原体中，并通过取代硫来破坏细胞结构。仅在25 ppm时就实现了对白色念珠菌生物膜50%的抑制。最后，证明对白色念珠菌生存能力有强烈影响的两个物理参数是结晶度和粒径。
