Hesabizadeh Tina, Sung Kidon, Park Miseon, Foley Steven, Paredes Angel, Blissett Stephen, Guisbiers Gregory
Department of Physics and Astronomy, University of Arkansas at Little Rock, 2801 South University Avenue, Little Rock, AR 72204, USA.
Division of Microbiology, National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA.
Nanomaterials (Basel). 2023 Jul 29;13(15):2206. doi: 10.3390/nano13152206.
Spherical copper oxide nanoparticles (CuO/CuO NPs) were synthesized by pulsed laser ablation in liquids (PLAL). The copper target was totally submerged in deionized (DI) water and irradiated by an infrared laser beam at 1064 nm for 30 min. The NPs were then characterized by dynamic light scattering (DLS) and atomic emission spectroscopy (AES) to determine their size distribution and concentration, respectively. The phases of copper oxide were identified by Raman spectroscopy. Then, the antibacterial activity of CuO/CuO NPs against foodborne pathogens, such as subsp. serotype Typhimurium DT7, O157:H7, ATCC 9290, ATCC 27729, ATCC 49398, ATCC 11778, and EGD, was tested. At a 3 ppm concentration, the CuO/CuO NPs exhibited an outstanding antimicrobial effect by killing most bacteria after 5 h incubation at 25 °C. Field emission scanning electron microscope (FESEM) confirmed that the CuO/CuO NPs destructed the bacterial cell wall.
通过液体中的脉冲激光烧蚀（PLAL）合成了球形氧化铜纳米颗粒（CuO/CuO NPs）。将铜靶完全浸没在去离子（DI）水中，并用波长为1064 nm的红外激光束照射30分钟。然后分别通过动态光散射（DLS）和原子发射光谱（AES）对纳米颗粒进行表征，以确定其尺寸分布和浓度。通过拉曼光谱鉴定氧化铜的相。然后，测试了CuO/CuO NPs对食源性病原体的抗菌活性，如鼠伤寒沙门氏菌亚种血清型Typhimurium DT7、O157:H7、ATCC 9290、ATCC 27729、ATCC 49398、ATCC 11778和EGD。在3 ppm的浓度下，CuO/CuO NPs在25°C孵育5小时后杀死了大多数细菌，表现出出色的抗菌效果。场发射扫描电子显微镜（FESEM）证实CuO/CuO NPs破坏了细菌细胞壁。
