Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Daru. 2014 Jun 6;22(1):48. doi: 10.1186/2008-2231-22-48.
Lactic acid bacteria are considered important probiotics for prevention of some infections. The aim of this work was to investigate the effect of selenium dioxide on the antifungal activity of Lactobacillus plantarum and L. johnsonii against Candida albicans.
Lactobacillus plantarum and L. johnsonii cells, grown in the presence and absence of selenium dioxide, and their cell-free spent culture media were tested for antifungal activity against C. albicans ATCC 14053 by a hole-plate diffusion method and a time-kill assay.
Both L. plantarum and L. johnsonii reduced selenium dioxide to cell-associated elemental selenium nanoparticles. The cell-free spent culture media, from both Lactobacillus species that had been grown with selenium dioxide for 48 h, showed enhanced antifungal activity against C. albicans. Enhanced antifungal activity of cell biomass against C. albicans was also observed in cultures grown with selenium dioxide.
Selenium dioxide-treated Lactobacillus spp. or their cell-free spent broth inhibited the growth of C. albicans and should be investigated for possible use in anti-Candida probiotic formulations in future.
乳酸菌被认为是预防某些感染的重要益生菌。本研究旨在探讨二氧化硒对植物乳杆菌和约翰逊乳杆菌抗白色念珠菌的抗真菌活性的影响。
采用孔板扩散法和时间杀伤试验，检测在有或没有二氧化硒存在的情况下生长的植物乳杆菌和约翰逊乳杆菌细胞及其无细胞的废弃培养物对白色念珠菌 ATCC 14053 的抗真菌活性。
植物乳杆菌和约翰逊乳杆菌均将二氧化硒还原为细胞相关的元素硒纳米颗粒。来自两种乳酸菌的无细胞废弃培养液，在与二氧化硒共培养 48 小时后，对白色念珠菌的抗真菌活性增强。在含二氧化硒的培养物中也观察到细胞生物量对白色念珠菌的增强的抗真菌活性。
经二氧化硒处理的乳杆菌属或其无细胞废弃发酵液抑制白色念珠菌的生长，在未来可能用于抗白色念珠菌益生菌制剂的研究。
