Sanguinetti Maurizio, Posteraro Brunella
Insititute of Micorbiology, Fondazione Policlinico Universitario A. Gemelli, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
Insitutue of Public Health (Section of Hygiene), Fondazione Policlinico Universitario A. Gemelli, IRCCS, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
J Fungi (Basel). 2018 Sep 15;4(3):110. doi: 10.3390/jof4030110.
Susceptibility testing of fungi against antifungal drugs commonly used for therapy is a key component of the care of patients with invasive fungal infections. Antifungal susceptibility testing (AFST) has progressed in recent decades to finally become standardized and available as both Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) reference methods and in commercial manual/automated phenotypic methods. In clinical practice, the Sensititre YeastOne and Etest methods are widely used for AFST, particularly for sterile site isolates of . Nevertheless, AFST is moving toward new phenotypic methods, such as matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), that are capable of providing rapid, and potentially more actionable, results for the treating clinician. Our objective is to summarize updated data on phenotypic methods for AFST of and species and to assess their significance in view of opposing, but emerging, molecular genotypic methods.
对常用于治疗的抗真菌药物进行真菌药敏试验是侵袭性真菌感染患者护理的关键组成部分。近几十年来，抗真菌药敏试验（AFST）取得了进展，最终实现了标准化，可作为临床和实验室标准协会（CLSI）及欧洲抗菌药物敏感性试验委员会（EUCAST）的参考方法，也有商业化的手动/自动化表型方法。在临床实践中，Sensititre YeastOne和Etest方法被广泛用于AFST，特别是对于无菌部位分离株。然而，AFST正在向新的表型方法发展，如基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS），它能够为治疗临床医生提供快速且可能更具可操作性的结果。我们的目标是总结关于曲霉属和念珠菌属物种AFST表型方法的最新数据，并鉴于相反但正在兴起的分子基因型方法评估其意义。
