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用于从人类粪便中分离推定的D群链球菌的选择性培养基的比较。

Comparison of selective media for isolation of presumptive group D streptococci from human feces.

作者信息

Sabbaj J, Sutter V L, Finegold S M

出版信息

Appl Microbiol. 1971 Dec;22(6):1008-11. doi: 10.1128/am.22.6.1008-1011.1971.

Abstract

Pfizer Selective Enterococcus (PSE) agar, a medium containing bile, sodium azide, and esculin, was evaluated for its sensitivity and selectivity for detection and enumeration of presumptive group D streptococci in human feces. SF broth and SF broth plus agar (1.5%), representing selective media in common use, were studied simultaneously. Presumptive group D streptococci were recovered on PSE agar from the feces of all 25 subjects. No growth was observed in 8% of specimens in SF broth. No gram-negative organisms were recovered in any medium. PSE agar has the advantages of selecting out Streptococcus bovis, earlier appearance of distinctive reactions, and lack of requirement for special incubation temperature.

摘要

辉瑞选择性肠球菌(PSE)琼脂是一种含有胆汁、叠氮化钠和七叶苷的培养基,对其在检测和计数人粪便中推定的D群链球菌时的敏感性和选择性进行了评估。同时研究了代表常用选择性培养基的SF肉汤和加有琼脂(1.5%)的SF肉汤。在PSE琼脂上从所有25名受试者的粪便中均分离出推定的D群链球菌。在SF肉汤中8%的标本未观察到生长。在任何培养基中均未分离出革兰氏阴性菌。PSE琼脂具有选出牛链球菌、独特反应出现较早以及无需特殊培养温度的优点。

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