Wang Songbo, Liang Xingwei, Yang Qiyuan, Fu Xing, Zhu Meijun, Rodgers B D, Jiang Qingyan, Dodson Michael V, Du Min
College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, P.R. China.
Department of Animal Sciences, Washington Center for Muscle Biology, Washington State University, Pullman, WA, USA.
Mol Nutr Food Res. 2017 Apr;61(4). doi: 10.1002/mnfr.201600746. Epub 2017 Jan 3.
Enhancing the formation and function of brown adipose tissue (BAT) increases thermogenesis and hence reduces obesity. Thus, we investigate the effects of resveratrol (Resv) on brown adipocyte formation and function in mouse interscapular BAT (iBAT).
CD1 mice and stromal vascular cells (SVCs) isolated from iBAT were treated with Resv. Expression of brown adipogenic and thermogenic markers, and involvement of AMP-activated protein kinase (AMPK)α1 were assessed. In vivo, Resv-enhanced expression of brown adipogenic markers, PR domain-containing 16 (PRDM16) and thermogenic genes, uncoupling protein 1 (UCP1) and cytochrome C in iBAT, along with smaller lipid droplets, elevated AMPKα activity and increased oxygen consumption. Meanwhile, Resv promoted expression of PRDM16, UCP1, PGC1α, cytochrome C and pyruvate dehydrogenase (PDH) in differentiated iBAT SVCs, suggesting that Resv enhanced brown adipocyte formation and function in vitro. In addition, Resv stimulated AMPKα and oxygen consumption in differentiated iBAT SVCs. However, the promotional effects of Resv were diminished by AMPK inhibition or AMPKα1 knockout, implying the involvement of AMPKα1 in this process.
Resv enhanced brown adipocyte formation and thermogenic function in mouse iBAT by promoting the expression of brown adipogenic markers via activating AMPKα1, which contributed to the anti-obesity effects of Resv.
增强棕色脂肪组织（BAT）的形成和功能可增加产热，从而减轻肥胖。因此，我们研究了白藜芦醇（Resv）对小鼠肩胛间棕色脂肪组织（iBAT）中棕色脂肪细胞形成和功能的影响。
用Resv处理CD1小鼠及从iBAT分离的基质血管细胞（SVCs）。评估棕色脂肪生成和产热标志物的表达以及AMP激活的蛋白激酶（AMPK）α1的参与情况。在体内，Resv增强了iBAT中棕色脂肪生成标志物含PR结构域的16（PRDM16）和产热基因解偶联蛋白1（UCP1）以及细胞色素C的表达，同时脂滴变小，AMPKα活性升高，氧消耗增加。同时，Resv促进了分化的iBAT SVCs中PRDM16、UCP1、PGC1α、细胞色素C和丙酮酸脱氢酶（PDH）的表达，表明Resv在体外增强了棕色脂肪细胞的形成和功能。此外，Resv刺激了分化的iBAT SVCs中的AMPKα和氧消耗。然而，Resv的促进作用因AMPK抑制或AMPKα1基因敲除而减弱，这意味着AMPKα1参与了这一过程。
Resv通过激活AMPKα1促进棕色脂肪生成标志物的表达，增强了小鼠iBAT中棕色脂肪细胞的形成和产热功能，这有助于Resv的抗肥胖作用。
