Xing Tingting, Liu Yongqin, Wang Ninglian, Xu Baiqing, Shen Liang, Liu Keshao, Gu Zhengquan, Guo Bixi, Zhou Yuguang, Liu Hongcan
Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.
Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2017 Mar;67(3):617-620. doi: 10.1099/ijsem.0.001672. Epub 2017 Apr 3.
A Gram-stain-negative, non-spore-forming, rod-shaped bacterium, B555-2, was isolated from an ice core drilled from Muztagh Glacier on the Tibetan Plateau, China. According to phylogenetic analysis with 16S rRNA gene sequences, the novel strain was most closely related to Polymorphobacter fuscus D40P and Polymorphobacter multimanifer 262-7 with 98.4 and 96.9 % similarity, respectively. It grew optimally at pH 7 and 15 °C with 0.6 % NaCl (m/v). Carotenoid was detected from the cells. Major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, sphingoglycolipid, phophatidylmonomethy lethanolamine, phophatidylcholine. The major fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) (42.8 %), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) (28.8 %), C14 : 0 2-OH (10.1 %) and C16 : 0 (8.2 %). The predominant ubiquinone was Q-10. The DNA G+C content was 62.1 mol%. In DNA-DNA hybridization tests, strain B555-2 shared 21.9 and 18.6 % DNA-DNA relatedness with P. fuscus D40P and P.multimanifer 262-7, respectively. Accordingly, strain B555-2 represents a novel species in the genus Polymorphobacter, for which the name Polymorphobacter glacialis sp. nov. is proposed. The type strain is B555-2 (=CGMCC 1.15519=KCTC 52396).
从中国青藏高原慕士塔格冰川钻取的冰芯中分离出一株革兰氏阴性、无芽孢、杆状细菌B555 - 2。基于16S rRNA基因序列的系统发育分析表明，该新菌株与暗褐多形杆菌D40P和多形多样杆菌262 - 7亲缘关系最近，相似性分别为98.4%和96.9%。其最适生长条件为pH 7、15℃，NaCl浓度为0.6%（m/v）。细胞中检测到类胡萝卜素。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、鞘糖脂、磷脂酰单甲基乙醇胺、磷脂酰胆碱。主要脂肪酸为总和特征3（C16 : 1ω7c和/或异-C15 : 0 2-OH）（42.8%）、总和特征8（C18 : 1ω7c和/或C18 : 1ω6c）（28.8%）、C14 : 0 2-OH（10.1%）和C16 : 0（8.2%）。主要泛醌为Q-10。DNA G+C含量为62.1 mol%。在DNA-DNA杂交试验中，菌株B555 - 2与暗褐多形杆菌D40P和多形多样杆菌262 - 7的DNA-DNA相关性分别为21.9%和18.6%。因此，菌株B555 - 2代表多形杆菌属中的一个新物种，提议将其命名为冰川多形杆菌（Polymorphobacter glacialis sp. nov.）。模式菌株为B555 - 2（=CGMCC 1.15519=KCTC 52396）。
