Department of Clinical Laboratory, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 510006, People's Republic of China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Arch Microbiol. 2021 Oct;203(8):5067-5074. doi: 10.1007/s00203-021-02454-9. Epub 2021 Jul 24.
Strain SZY PN-1, representing a novel Gram-negative, aerobic, non-motile, rod-shaped and yellow-pigmented bacterium, was isolated from a skin sample of a healthy Chinese male. Growth occurred at pH 6.0-8.0 (optimum, pH 7.0) and 10-37 ℃ (optimum, 30 ℃) with 0-1.0% (w/v) NaCl in R2A agar. Comparative analysis of the 16S rRNA gene sequences revealed that strain SZY PN-1 shared high similarities with two invalid-published species, "Sandaracinobacter sibiricus" RB16-17 (97.1%) and "Sandaracinobacter neustonicus" JCM 30734 (96.6%), respectively. Phylogenetic analysis of 16S rRNA gene sequences together with protein-concatemer tree showed that SZY PN-1 formed a separate branch within the family Sphingosinicellaceae. The DNA G + C content of the strain SZY PN-1 was 65.0% (genome). The polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, two sphingoglycolipids, diphosphatidylglycerol, five unidentified glycolipids, and seven unidentified lipids. The predominant fatty acids (> 10.0%) were identified as C ω7c and/or C ω6c, C ω6c, C ω7c and/or C ω6c. The major respiratory quinone was Q-10. Based on the phenotypic and genotypic features, a novel genus and species, Sandaracinobacteroides hominis gen. nov., sp. nov. is proposed, with type strain SZY PN-1 (= KCTC 82150 = NBRC 114675).
SZY PN-1 菌株，代表一种新型革兰氏阴性、需氧、非运动、杆状和黄色素产生菌，从一名健康中国男性的皮肤样本中分离得到。该菌在 R2A 琼脂中于 pH6.0-8.0（最佳，pH7.0）和 10-37℃（最佳，30℃）、0-1.0%（w/v）NaCl 下生长。16S rRNA 基因序列的比较分析表明，菌株 SZY PN-1 与两个无效发表的种“沙达沙伯菌”RB16-17（97.1%）和“海栖沙达沙伯菌”JCM 30734（96.6%）具有高度相似性。16S rRNA 基因序列的系统发育分析以及蛋白凝聚树分析表明，SZY PN-1 在鞘脂单胞菌科内形成一个单独的分支。该菌株的 DNA G+C 含量为 65.0%（基因组）。极性脂谱包括磷脂酰乙醇胺、磷脂酰甘油、两种神经酰胺糖脂、双磷脂酰甘油、五种未知糖脂和七种未知脂类。主要脂肪酸（>10.0%）鉴定为 C ω7c 和/或 C ω6c、C ω6c、C ω7c 和/或 C ω6c。主要呼吸醌为 Q-10。基于表型和基因型特征，提出了一个新属和新种，海栖沙达沙伯菌属 homo 种，新种，模式菌株为 SZY PN-1（=KCTC 82150= NBRC 114675）。
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