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新种,包含 ML 光合作用基因,从火山湖中分离得到。

sp. nov., containing the photosynthetic gene ML, isolated from a volcanic lake.

机构信息

China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.

Tibet Plateau Institute of Biology, Lhasa, Tibet 850001, PR China.

出版信息

Int J Syst Evol Microbiol. 2020 Feb;70(2):1093-1098. doi: 10.1099/ijsem.0.003880.

Abstract

An aerobic, Gram-stain-negative, yellowish, rod-shaped bacterium, designated DJ1R-1, was isolated from water sample from a volcanic lake, located on Da Hinggan Ling Mountain, PR China. Growth of DJ1R-1 optimally occurred at pH 7.0, at 22-25 °C and with 0-0.5 % (w/v) NaCl concentration. Phylogenetic analysis of 16S rRNA gene sequences indicated that DJ1R-1 was clustered into the genus and showed 96.5 %, 95.9 % and 95.6 % similarities to D40P, 262-7 and B555-2, respectively. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, three unidentified aminolipids and one unidentified phospholipid. The major fatty acids were summed feature 8 (Cω7 / Cω6, 40.0 %), summed feature 3 (Cω7 / Cω6, 25.6 %) and C (13.7 %). The respiratory quinone was ubiquinone-10. The DNA G+C content was 65.0 % according to the genomic sequencing results. On the basis of the results of the phylogenetic analysis, physiological and biochemical properties comparisons, DJ1R-1 was proposed to represent a novel species of the genus , with the name . The type strain is DJ1R-1 (=CGMCC 1.13788=KCTC 72014).

摘要

一株好氧、革兰氏阴性、浅黄色、杆状细菌,被命名为 DJ1R-1,从位于中国大兴安岭的火山湖水样中分离得到。DJ1R-1 的最佳生长条件为 pH 值 7.0、22-25°C 和 0-0.5%(w/v)NaCl 浓度。16S rRNA 基因序列的系统发育分析表明,DJ1R-1 属于 属,与 D40P、262-7 和 B555-2 的相似性分别为 96.5%、95.9%和 95.6%。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基磷脂、三种未鉴定的氨基脂和一种未鉴定的磷脂。主要脂肪酸为 sum feature 8(Cω7/Cω6,40.0%)、sum feature 3(Cω7/Cω6,25.6%)和 C(13.7%)。呼吸醌为泛醌-10。根据基因组测序结果,DNA G+C 含量为 65.0%。基于系统发育分析、生理生化特性比较的结果,DJ1R-1 被提议代表 属的一个新种,命名为. 模式菌株为 DJ1R-1(=CGMCC 1.13788=KCTC 72014)。

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