China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Tibet Plateau Institute of Biology, Lhasa, Tibet 850001, PR China.
Int J Syst Evol Microbiol. 2020 Feb;70(2):1093-1098. doi: 10.1099/ijsem.0.003880.
An aerobic, Gram-stain-negative, yellowish, rod-shaped bacterium, designated DJ1R-1, was isolated from water sample from a volcanic lake, located on Da Hinggan Ling Mountain, PR China. Growth of DJ1R-1 optimally occurred at pH 7.0, at 22-25 °C and with 0-0.5 % (w/v) NaCl concentration. Phylogenetic analysis of 16S rRNA gene sequences indicated that DJ1R-1 was clustered into the genus and showed 96.5 %, 95.9 % and 95.6 % similarities to D40P, 262-7 and B555-2, respectively. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, three unidentified aminolipids and one unidentified phospholipid. The major fatty acids were summed feature 8 (Cω7 / Cω6, 40.0 %), summed feature 3 (Cω7 / Cω6, 25.6 %) and C (13.7 %). The respiratory quinone was ubiquinone-10. The DNA G+C content was 65.0 % according to the genomic sequencing results. On the basis of the results of the phylogenetic analysis, physiological and biochemical properties comparisons, DJ1R-1 was proposed to represent a novel species of the genus , with the name . The type strain is DJ1R-1 (=CGMCC 1.13788=KCTC 72014).
一株好氧、革兰氏阴性、浅黄色、杆状细菌，被命名为 DJ1R-1，从位于中国大兴安岭的火山湖水样中分离得到。DJ1R-1 的最佳生长条件为 pH 值 7.0、22-25°C 和 0-0.5%（w/v）NaCl 浓度。16S rRNA 基因序列的系统发育分析表明，DJ1R-1 属于 属，与 D40P、262-7 和 B555-2 的相似性分别为 96.5%、95.9%和 95.6%。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基磷脂、三种未鉴定的氨基脂和一种未鉴定的磷脂。主要脂肪酸为 sum feature 8（Cω7/Cω6，40.0%）、sum feature 3（Cω7/Cω6，25.6%）和 C（13.7%）。呼吸醌为泛醌-10。根据基因组测序结果，DNA G+C 含量为 65.0%。基于系统发育分析、生理生化特性比较的结果，DJ1R-1 被提议代表 属的一个新种，命名为. 模式菌株为 DJ1R-1（=CGMCC 1.13788=KCTC 72014）。
