China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004886.
A novel Gram-stain-negative, rod-shaped, yellow bacterium, designated as LB1R16, was isolated from the Laigu glacier on the Tibetan Plateau, PR China. Strain LB1R16 was catalase-positive, oxidase-negative and grew at 0-28 °C, pH 6.0-8.0 and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LB1R16 belongs to the family but formed an independent lineage. The highest level of 16S rRNA gene sequence similarities were found to DJ1R-1 (95.24 %), YLT33 (94.78 %) and DSM 17366 (94.67 %). The genomic DNA G+C content was 68.8 mol%. The main cellular fatty acids were summed feature 8 (C 7/C 6), summed feature 3 (C 7/C 6), C and C-OH. The respiratory quinone was ubiquinone-10. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one sphingoglycolipid, one unidentified aminolipid, one unidentified phospholipid and two unidentified polar lipids, which were different from the type strains of , and . Based on a polyphasic approach, a novel species of a new genus, gen. nov., sp. nov., within the family is proposed. The type strain is LB1R16 (=CGMCC 1.11941=NBRC 113873).
一株新型革兰氏阴性、杆状、黄色细菌，命名为 LB1R16，从中国青藏高原的来古冰川中分离得到。菌株 LB1R16 为过氧化氢酶阳性、氧化酶阴性，可在 0-28°C、pH 值 6.0-8.0 和无 NaCl 的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 LB1R16 属于 科，但形成一个独立的进化枝。与 DJ1R-1（95.24%）、YLT33（94.78%）和 DSM 17366（94.67%）的 16S rRNA 基因序列相似度最高。基因组 DNA G+C 含量为 68.8mol%。主要细胞脂肪酸为 sumFeature8（C7/C6）、sumFeature3（C7/C6）、C 和 C-OH。呼吸醌为泛醌-10。极性脂包括磷脂酰乙醇胺、磷脂酰甘油、一种神经酰胺糖脂、一种未鉴定的胺脂、一种未鉴定的磷脂和两种未鉴定的极性脂，与 科的模式菌株不同 。基于多相分类法，提出了一个新属、一个新种，属于 科，命名为 属，新种，命名为 LB1R16（=CGMCC 1.11941=NBRC 113873）。
