Graduate Program in Genetics and Molecular Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.
Division of Microbial Resources, Research Center for Agriculture, Biological and Chemical, University of Campinas, Paulínia, SP, Brazil.
Sci Rep. 2024 Jun 21;14(1):14285. doi: 10.1038/s41598-024-64349-1.
A polyphasic approach was applied to characterize taxonomically a novel endophytic bacterial strain, designated as EP178, which was previously isolated from Passiflora incarnata leaves and characterized as plant-growth promoter. The strain EP178 forms Gram stain-negative and rod-shaped cells, and circular and yellow-pigmented colonies. Its growth occurs at 10-37 °C, at pH 6.0-8.0, and tolerates up to 7% (w/v) NaCl. The major cellular fatty acids found were summed feature 8 (C ω7c), summed feature 3 (C ω6c /C ω7c), and C, and the predominant ubiquinone was Q-9. The phylogenetic and nucleotide-similarity analysis with 16S rRNA gene sequences showed that strain EP178 belongs to Pseudomonas genus. The genomic-based G + C content was 65.5%. The average nucleotide identity and digital DNA-DNA hybridization values between strains EP178 and the closest type strain, P. oryzihabitans DSM 6835, were 92.6% and 52.2%, respectively. Various genes associated with plant-growth promoting mechanisms were annotated from genome sequences. Based on the phenotypic, genomic, phylogeny and chemotaxonomic data, strain EP178 represents a new species of the genus Pseudomonas, for which the name Pseudomonas flavocrustae sp. nov. was proposed. The type strain is EP178 (= CBMAI 2609 = ICMP 24844 = MUM 23.01).
采用多相分类法对先前从穿心莲叶中分离出的、具有促植物生长特性的新型内生细菌菌株 EP178 进行了分类学表征。菌株 EP178 呈革兰氏阴性、杆状细胞，圆形、黄色菌落。其生长温度为 10-37°C，pH 值为 6.0-8.0，可耐受高达 7%（w/v）的 NaCl。主要细胞脂肪酸为总和特征 8（C ω7c）、总和特征 3（C ω6c /C ω7c）和 C，主要的类异戊二烯醌为 Q-9。16S rRNA 基因序列的系统发育和核苷酸相似性分析表明，菌株 EP178 属于假单胞菌属。基于基因组的 G+C 含量为 65.5%。菌株 EP178 和最接近的模式菌株 P. oryzihabitans DSM 6835 之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 92.6%和 52.2%。从基因组序列中注释了与促植物生长机制相关的各种基因。基于表型、基因组、系统发育和化学分类学数据，菌株 EP178 代表了假单胞菌属的一个新种，拟名为 Pseudomonas flavocrustae sp. nov.。模式菌株为 EP178（=CBMAI 2609=ICMP 24844=MUM 23.01）。
