Sharifi Fereshteh, Irani Shiva, Zandi Mojgan, Soleimani Masoud, Atyabi Seyed Mohammad
Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, Iran.
Prog Biomater. 2016 Dec;5(3-4):213-222. doi: 10.1007/s40204-016-0059-1. Epub 2016 Dec 8.
One of the determinant factors for successful bioengineering is to achieve appropriate nano-topography and three-dimensional substrate. In this research, polycaprolactone (PCL) nano-fibrous mat with different roughness modified with O plasma was fabricated via electrospinning. The purpose of this study was to evaluate the effect of plasma modification along with surface nano-topography of mats on the quality of human fibroblast (HDFs) and osteoblast cells (OSTs)-substrate interaction. Surface properties were studied using scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle, Fourier-transformation infrared spectroscopy. We evaluated mechanical properties of fabricated mats by tensile test. The viability and proliferation of HDFs and OSTs on the substrates were followed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT). Mineralization of the substrate was determined by alizarin red staining method and calcium content of OSTs was determined by calcium content kit. Cells morphology was studied by SEM analysis. The results revealed that the plasma-treated electrospun nano-fibrous substrate with higher roughness was an excellent designed substrate. A bioactive topography for stimulating proliferation of HDFs and OSTs is to accelerate the latter's differentiation time. Therefore, the PCL substrate with high density and major nano-topography were considered as a bio-functional and elegant bio-substrate for tissue regeneration applications.
生物工程成功的决定性因素之一是实现合适的纳米拓扑结构和三维基质。在本研究中，通过静电纺丝制备了经氧等离子体改性的具有不同粗糙度的聚己内酯（PCL）纳米纤维垫。本研究的目的是评估等离子体改性以及垫的表面纳米拓扑结构对人成纤维细胞（HDFs）和成骨细胞（OSTs）与基质相互作用质量的影响。使用扫描电子显微镜（SEM）、原子力显微镜（AFM）、接触角、傅里叶变换红外光谱研究表面性质。通过拉伸试验评估制备垫的力学性能。用3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）跟踪HDFs和OSTs在基质上的活力和增殖。通过茜素红染色法测定基质的矿化程度，并用钙含量试剂盒测定OSTs的钙含量。通过SEM分析研究细胞形态。结果表明，经等离子体处理的具有较高粗糙度的静电纺纳米纤维基质是一种设计优良的基质。刺激HDFs和OSTs增殖的生物活性拓扑结构可加速后者的分化时间。因此，具有高密度和主要纳米拓扑结构的PCL基质被认为是用于组织再生应用的生物功能且优良的生物基质。
