Tong Yuehong, Ben Ami Tal, Hong Sungmin, Heintzmann Rainer, Gerig Guido, Ablonczy Zsolt, Curcio Christine A, Ach Thomas, Smith R Theodore
*Department of Ophthalmology, New York University School of Medicine, New York, New York; †Department of Computer Science and Engineering, New York University Tandon School of Engineering, Brooklyn, New York; ‡Leibniz Institute of Photonic Technology, Jena, Germany; §Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University of Jena, Jena, Germany; ¶Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina; **Department of Ophthalmology, University of Alabama School of Medicine, Birmingham, Alabama; and ††Department of Ophthalmology, University Hospital of Würzburg, Würzburg, Germany.
Retina. 2016 Dec;36 Suppl 1(Suppl 1):S127-S136. doi: 10.1097/IAE.0000000000001325.
To elucidate the molecular pathogenesis of age-related macular degeneration (AMD) and interpretation of fundus autofluorescence imaging, the authors identified spectral autofluorescence characteristics of drusen and retinal pigment epithelium (RPE) in donor eyes with AMD.
Macular RPE/Bruch membrane flat mounts were prepared from 5 donor eyes with AMD. In 12 locations (1-3 per eye), hyperspectral autofluorescence images in 10-nm-wavelength steps were acquired at 2 excitation wavelengths (λex 436, 480 nm). A nonnegative tensor factorization algorithm was used to recover 5 abundant emission spectra and their corresponding spatial localizations.
At λex 436 nm, the authors consistently localized a novel spectrum (SDr) with a peak emission near 510 nm in drusen and sub-RPE deposits. Abundant emission spectra seen previously (S0 in Bruch membrane and S1, S2, and S3 in RPE lipofuscin/melanolipofuscin, respectively) also appeared in AMD eyes, with the same shapes and peak wavelengths as in normal tissue. Lipofuscin/melanolipofuscin spectra localizations in AMD eyes varied widely in their overlap with drusen, ranging from none to complete.
An emission spectrum peaking at ∼510 nm (λex 436 nm) appears to be sensitive and specific for drusen and sub-RPE deposits. One or more abundant spectra from RPE organelles exhibit characteristic relationships with drusen.
为阐明年龄相关性黄斑变性（AMD）的分子发病机制并解读眼底自发荧光成像，作者确定了患有AMD的供体眼中玻璃膜疣和视网膜色素上皮（RPE）的光谱自发荧光特征。
从5只患有AMD的供体眼中制备黄斑RPE/ Bruch膜平铺标本。在12个位置（每只眼1 - 3个位置），以10纳米波长步长在2个激发波长（λex 436、480纳米）下采集高光谱自发荧光图像。使用非负张量分解算法恢复5种丰富的发射光谱及其相应的空间定位。
在λex 436纳米时，作者始终在玻璃膜疣和RPE下沉积物中定位到一种新的光谱（SDr），其发射峰值接近510纳米。先前观察到的丰富发射光谱（Bruch膜中的S0以及RPE脂褐素/黑素脂褐素中的S1、S2和S3）在AMD眼中也出现，其形状和峰值波长与正常组织相同。AMD眼中脂褐素/黑素脂褐素光谱定位与玻璃膜疣的重叠程度差异很大，从无重叠到完全重叠。
在约510纳米（λex 436纳米）处达到峰值的发射光谱似乎对玻璃膜疣和RPE下沉积物敏感且具有特异性。来自RPE细胞器的一种或多种丰富光谱与玻璃膜疣呈现出特征性关系。
