犬肠道病相关大细胞型T细胞淋巴瘤患者来源异种移植模型的建立
Establishment of a Patient-Derived Xenograft of Canine Enteropathy-Associated T-Cell Lymphoma, Large Cell Type.
作者信息
Im K S, Kim J H, Graef A J, Cornax I, Seelig D M, O'Sullivan M G, Kovi R C, Modiano J F
机构信息
Animal Cancer Care and Research Program, University of Minnesota, St. Paul, USA; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, USA; Masonic Cancer Center, University of Minnesota, Minneapolis, USA; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, USA.
Animal Cancer Care and Research Program, University of Minnesota, St. Paul, USA; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, USA; Masonic Cancer Center, University of Minnesota, Minneapolis, USA.
出版信息
J Comp Pathol. 2017 Jan;156(1):37-41. doi: 10.1016/j.jcpa.2016.11.271. Epub 2016 Dec 20.
The pathogenesis of canine T-cell lymphoma remains incompletely understood, partly because there are no well-established in-vivo models to study these malignancies. For this study, we generated a patient-derived tumour xenograft (PDTX) from a 10-year-old neutered male golden retriever dog with enteropathy-associated intestinal T-cell lymphoma, large cell type. One of two female, 15-week-old beige/nude/XID mice developed a visible tumour 7 weeks after sections of tumour material from the spleen were surgically implanted. The histological appearance, immunophenotype and clonal antigen receptor rearrangements of the tumour from the recipient mouse showed that it was derived from the primary canine tumour. Our results indicate that immunodeficient mice are receptive hosts to develop in-vivo PDTX models to study the pathogenesis and management of canine T-cell lymphomas.
犬T细胞淋巴瘤的发病机制仍未完全明确，部分原因是缺乏成熟的体内模型来研究这些恶性肿瘤。在本研究中，我们从一只10岁已绝育的雄性金毛猎犬身上获取了患者来源的肿瘤异种移植模型（PDTX），该犬患有肠病相关的大细胞型肠道T细胞淋巴瘤。在将脾脏肿瘤组织切片手术植入两只15周龄雌性米色/裸/XID小鼠中的一只后，7周时出现了可见肿瘤。受体小鼠肿瘤的组织学表现、免疫表型和克隆性抗原受体重排表明，它源自原发性犬肿瘤。我们的结果表明，免疫缺陷小鼠是建立体内PDTX模型以研究犬T细胞淋巴瘤发病机制和治疗的适宜宿主。
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