Tabba H D, Chang R S, Smith K M
Department of Chemistry, University of California, Davis 95616.
Antiviral Res. 1989 Jun-Jul;11(5-6):263-73. doi: 10.1016/0166-3542(89)90036-3.
Prunellin, an anti-HIV active compound, was isolated from aqueous extracts of the Chinese medicinal herb, Prunella vulgaris, and purified to chromatographic homogeneity. Infrared and NMR spectroscopy identified prunellin as a polysaccharide. Elemental analyses, precipitation with calcium(II), barium(II), or 9-aminoacridine suggest a sulfated polysaccharide. Paper chromatography of the exhaustively hydrolyzed material indicates the presence of glucose, galactose, xylose, gluconic acid, galactonic acid and galactosamine as the constituent monosaccharides. The molecular size of prunellin, as determined by gel permeation chromatography and the Squire method on Sephadex G-75, is about 10 kDa.
夏枯草素是一种具有抗HIV活性的化合物，它是从中药夏枯草的水提取物中分离出来的，并通过色谱法纯化至均一。红外光谱和核磁共振光谱鉴定夏枯草素为一种多糖。元素分析、用钙（II）、钡（II）或9-氨基吖啶沉淀表明它是一种硫酸化多糖。对完全水解后的物质进行纸色谱分析表明，其组成单糖有葡萄糖、半乳糖、木糖、葡萄糖酸、半乳糖酸和半乳糖胺。通过凝胶渗透色谱法和在Sephadex G-75上的斯奎尔方法测定，夏枯草素的分子大小约为10 kDa。
