Lee S, Kweon O-K, Kim W H
Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Korea.
J Vet Intern Med. 2017 Jan;31(1):36-42. doi: 10.1111/jvim.14612. Epub 2016 Dec 29.
Leptin and its receptor play a role in several disease processes such as pancreatitis and heart disease. However, their association with gallbladder mucocele (GBM) in dogs has not been reported.
HYPOTHESIS/OBJECTIVES: To evaluate differences in the expression of leptin and leptin receptor between dogs with and without GBM.
Twenty-five healthy dogs, including 9 laboratory beagle dogs, and 22 client-owned dogs with GBM.
Serum leptin concentration was determined in blood samples of all dogs by ELISA. Canine gallbladder samples were collected from 9 dogs with GBM that underwent surgery for therapeutic purposes and from 9 healthy laboratory beagle dogs as a normal control group. Samples were analyzed for leptin and leptin receptor mRNA by real-time polymerase chain reaction.
Serum leptin concentration was significantly higher in dogs with GBM than in healthy dogs (medians of 7.03 and 2.18 ng/mL, respectively; P < .001). Patients with GBM that had undergone surgery had significantly higher serum leptin concentrations than those that had not (medians of 12.2 and 4.09 ng/mL, respectively; P = .001). However, no difference in serum leptin concentration was found between dogs with GBM with or without endocrinopathies. The mRNA expression levels of leptin and its receptor were significantly increased in the gallbladder tissues of dogs with GBM.
Dysregulation of leptin might be involved in the pathophysiology of GBM, and leptin concentrations might be associated with GBM severity.
瘦素及其受体在胰腺炎和心脏病等多种疾病过程中发挥作用。然而，它们与犬胆囊黏液囊肿（GBM）的关联尚未见报道。
假设/目的：评估患有和未患有GBM的犬之间瘦素和瘦素受体表达的差异。
25只健康犬，包括9只实验用比格犬，以及22只患有GBM的客户拥有的犬。
通过ELISA测定所有犬血液样本中的血清瘦素浓度。从9只因治疗目的接受手术的患有GBM的犬以及9只健康实验用比格犬作为正常对照组收集犬胆囊样本。通过实时聚合酶链反应分析样本中的瘦素和瘦素受体mRNA。
患有GBM的犬血清瘦素浓度显著高于健康犬（中位数分别为7.03和2.18 ng/mL；P <.001）。接受手术的GBM患者血清瘦素浓度显著高于未接受手术的患者（中位数分别为12.2和4.09 ng/mL；P =.001）。然而，患有或未患有内分泌疾病的GBM犬之间血清瘦素浓度没有差异。GBM犬胆囊组织中瘦素及其受体的mRNA表达水平显著升高。
瘦素失调可能参与GBM的病理生理过程，且瘦素浓度可能与GBM严重程度相关。
