Lee Sungin, Kweon Oh-Kyeong, Kim Wan Hee
Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Republic of Korea.
PLoS One. 2017 Oct 31;12(10):e0187315. doi: 10.1371/journal.pone.0187315. eCollection 2017.
Leptin and its receptor play several physiological roles in the canine gallbladder, and the dysregulation of leptin might play a role in the pathogenesis of gallbladder diseases such as gallbladder mucocele. Previous studies revealed a positive association between hyperlipidemia and gallstones in humans. However, the latter is still unclear in dogs with cholelithiasis. In this study, we examined the differences in leptin, leptin receptor, total cholesterol, and triglyceride levels between healthy dogs and dogs with cholelithiasis, and evaluated the correlation between leptin and hyperlipidemia. Twenty-eight healthy dogs and 34 client-owned dogs with cholelithiasis were enrolled in the study. Leptin concentrations and lipid profiles were determined from sera, and leptin and leptin receptor expression levels were quantified in gallbladder tissue. In dogs with cholelithiasis, serum concentrations of leptin (p < 0.001), total cholesterol (p < 0.001), and triglycerides (p < 0.001) were significantly higher compared with those in healthy dogs. Positive correlations were observed between serum leptin and total cholesterol (95% confidence interval (CI) = 0.61-0.89, r = 0.725, p < 0.001), and between leptin and triglycerides (95% CI = 0.63-0.89, r = 0.782, p < 0.001) in the cholelithiasis group. Hypercholesterolemia (Odds Ratio (OR) = 9.720; 95% CI = 1.148-82.318) and hypertriglyceridemia (OR = 12.913; 95% CI = 1.548-107.722) were shown to be risk factors for gallstone disease. In cholelithiasis patients who underwent cholecystectomy, serum leptin levels were significantly higher than in patients that had not undergone surgery (p < 0.001). Leptin and leptin receptor expression was upregulated in the gallbladder tissues of cholelithiasis patients (p < 0.01 and p < 0.001, respectively). These results indicate that increased serum leptin concentrations and hyperlipidemia (hypercholesterolemia or hypertriglyceridemia) are associated with canine cholelithiasis and that homeostatic imbalance of these parameters might affect the pathogenesis of gallstones.
瘦素及其受体在犬胆囊中发挥多种生理作用，瘦素失调可能在胆囊疾病（如胆囊黏液囊肿）的发病机制中起作用。先前的研究揭示了人类高脂血症与胆结石之间存在正相关。然而，在患有胆结石的犬中，后者仍不清楚。在本研究中，我们检查了健康犬和患有胆结石的犬之间瘦素、瘦素受体、总胆固醇和甘油三酯水平的差异，并评估了瘦素与高脂血症之间的相关性。28只健康犬和34只客户拥有的患有胆结石的犬被纳入研究。从血清中测定瘦素浓度和血脂谱，并对胆囊组织中的瘦素和瘦素受体表达水平进行定量。在患有胆结石的犬中，与健康犬相比，血清瘦素（p < 0.001）、总胆固醇（p < 0.001）和甘油三酯（p < 0.001）浓度显著更高。在胆结石组中，观察到血清瘦素与总胆固醇之间呈正相关（95%置信区间（CI）= 0.61 - 0.89，r = 0.725，p < 0.001），以及瘦素与甘油三酯之间呈正相关（95% CI = 0.63 - 0.89，r = 0.782，p < 0.001）。高胆固醇血症（优势比（OR）= 9.720；95% CI = 1.148 - 82.318）和高甘油三酯血症（OR = 12.913；95% CI = 1.548 - 107.722）被证明是胆结石疾病的危险因素。在接受胆囊切除术的胆结石患者中，血清瘦素水平显著高于未接受手术的患者（p < 0.001）。瘦素和瘦素受体表达在胆结石患者的胆囊组织中上调（分别为p < 0.01和p < 0.001）。这些结果表明，血清瘦素浓度升高和高脂血症（高胆固醇血症或高甘油三酯血症）与犬胆结石有关，并且这些参数的稳态失衡可能影响胆结石的发病机制。
