Faes Katrien, Lahoutte Tony, Hoorens Anne, Tournaye Herman, Goossens Ellen
Biology of the testis (BITE), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090 Brussels, Belgium.
In vivo Cellular and Molecular Imaging laboratory, Vrije Universiteit Brussel (VUB), 1090 Brussels, Belgium; Nuclear Medicine Department, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel (VUB), 1090 Brussels, Belgium.
Reprod Biomed Online. 2017 Mar;34(3):291-297. doi: 10.1016/j.rbmo.2016.12.007. Epub 2016 Dec 15.
When fertility is impaired by anticancer treatment, spermatogonial stem cell transplantation (SSCT) could be used as a fertility restoration technique later on in life. Previously, we have demonstrated that a testicular cell suspension could be injected into a human cadaver testis, however, leakage to the interstitium was observed. In this study, injection of mouse testicular cells at an injection height of 50 cm (hydrostatic pressure) or via an automated injection pump (1400 µl, 2600 µl and 3000 µl) was evaluated. Significant difference in the filled radioactive volume was reached between the group in which 1400 µl was injected with an infusion pump and the groups in which 2600 µl (P = 0.019) or 3000 µl (P = 0.010) was injected. In all experimental groups green fluorescent protein positive (GFP) cells were observed in the seminiferous tubules. In conclusion, a lower injection height did not resolve the leakage of the injected cells to the interstitium. Using the infusion pump resulted in more efficient filling of the seminiferous tubules with lower interexperimental variability. Although leakage to the interstitium was still observed, with further optimisation, the use of an infusion pump for clinical application is advantageous.
当抗癌治疗损害生育能力时，精原干细胞移植（SSCT）可在日后作为一种恢复生育能力的技术使用。此前，我们已证明可将睾丸细胞悬液注入人体尸体睾丸，但观察到有细胞漏入间质。在本研究中，评估了在50厘米注射高度（静水压力）下或通过自动注射泵（分别注射1400微升、2600微升和3000微升）注射小鼠睾丸细胞的情况。在使用输液泵注射1400微升的组与注射2600微升（P = 0.019）或3000微升（P = 0.010）的组之间，填充的放射性体积存在显著差异。在所有实验组的生精小管中均观察到绿色荧光蛋白阳性（GFP）细胞。总之，较低的注射高度并不能解决注射细胞漏入间质的问题。使用输液泵可使生精小管填充更有效，且实验间变异性更低。尽管仍观察到有细胞漏入间质，但经过进一步优化，使用输液泵用于临床应用是有利的。
