Tavassoli-Hojjati Sara, Sattari Mandana, Ghasemi Tayebeh, Ahmadi Rahil, Mashayekhi Abbas
Department of Pediatric Dentistry, Dental School, Islamic Azad University, Tehran Branch, Tehran, Iran.
Department of Immunology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Eur J Dent. 2016 Oct-Dec;10(4):469-474. doi: 10.4103/1305-7456.195165.
The purpose of this study was to evaluate the effect of different concentrations of platelet-rich plasma (PRP) on the proliferation of undifferentiated periodontal ligament (PDL) fibroblasts.
The undifferentiated PDL fibroblasts were obtained from two healthy human premolar teeth and cultured in Dulbecco's modified Eagle's medium. Cell wells were divided into five groups. Experimental groups received 0.1%, 5%, or 50% PRP; the positive and negative control groups were cultured in fetal bovine serum (FBS) 12% and in a medium without FBS 12%, respectively. The plates were incubated at 37°C for 1, 2, 3, 4, and 7 days. PDL cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide proliferation assay. Statistical analysis of the data was accomplished using repeated measure ANOVA and Tukey's test. < 0.05 was considered statistically significant.
The 5% PRP had the greatest effect on undifferentiated fibroblast proliferation, which was significant on the 3 day. There was no significant difference between 0.1% PRP and positive control during the first 3 days. The group with 50% PRP presented significantly lower proliferation, compared to other experimental and control groups.
It may be concluded that the growth-stimulating effect of PRP is dose dependent with the best results in low concentrations.
本研究旨在评估不同浓度富血小板血浆（PRP）对未分化牙周膜（PDL）成纤维细胞增殖的影响。
从未分化的两颗健康人前磨牙获取PDL成纤维细胞，并在杜氏改良 Eagle 培养基中培养。细胞孔分为五组。实验组分别接受0.1%、5%或50%的PRP；阳性和阴性对照组分别在含12%胎牛血清（FBS）的培养基和不含12%FBS的培养基中培养。将培养板在37°C孵育1、2、3、4和7天。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐增殖试验评估PDL细胞活力。使用重复测量方差分析和Tukey检验对数据进行统计分析。P < 0.05被认为具有统计学意义。
5%的PRP对未分化成纤维细胞增殖的影响最大，在第3天具有显著性。在前3天，0.1%的PRP与阳性对照组之间无显著差异。与其他实验组和对照组相比，50%PRP组的增殖显著较低。
可以得出结论，PRP的促生长作用呈剂量依赖性，低浓度时效果最佳。
