Jain Supreet, Rathod Nirav, Nagi Ravleen, Sur Jaideep, Laheji Afshan, Gupta Naveen, Agrawal Priyanka, Prasad Swati
Senior Lecturer, Department of Oral Medicine and Radiology, New Horizon Dental College and Research Institute , Bilaspur, Chhattisgarh, India .
Assistant Professor, Department of Prosthodontics, Chandulal Memorial Medical College , Bhilai, Chhattisgarh, India .
J Clin Diagn Res. 2016 Nov;10(11):ZC41-ZC44. doi: 10.7860/JCDR/2016/21450.8890. Epub 2016 Nov 1.
Natural herbal remedies have shown promising anti-microbial property and fewer side effects compared to synthetic anti-microbial therapy. Aloe Vera is a medicinal plant used for management of various infections since ancient times as it has anti-inflammatory, anti-microbial, and immune-boosting properties.
The aim of the present study was to determine the anti-microbial and inhibitory activities of various concentration of Aloe Vera Gel (AVG) against oral pathogenic bacteria.
Subgingival calculus and aspiration of periapical abscess and periodontal abscess was done in 20 patients and the sample transferred to thioglycolate broth, which was incubated in Mutans Sanguis agar, blood agar and cultured in anaerobic gas chamber. The colonies formed were identified further by gram staining methods and biochemical fermentation tests (IMViC). Each isolated colony of identified bacteria were cultured separately in Muller-Hilton broth and incubated at 37°C for 24 hours. Anti-microbial activity of the AVG was tested by the disc diffusion method and minimum inhibitory concentration was determined by broth micro-dilution method.
Various staining and biochemical tests confirmed that the sample contained (), (), () and (). AVG showed anti-bacterial property at 100% and 50% concentration ('t' value = 7.504, p-value <0.001). At lower concentration there was no effect against the bacteria. At 100% AVG concentration, zone of inhibition measured was 6.9mm in , 6.3mm in , 6.8mm in and 6.6mm in . The standard drugs were also used to compare anti-bacterial property of AVG. Result showed that higher concentration (100%, 50%) of AVG has comparable zone of inhibition with Ofloxacin (5mcg) and Ciprofloxacin (30mcg).
AVG at higher concentration showed anti-bacterial property and can be used as a promising adjunct for oral health care.
与合成抗菌疗法相比，天然草药疗法已显示出有前景的抗菌特性且副作用较少。芦荟是一种药用植物，自古以来就用于治疗各种感染，因为它具有抗炎、抗菌和增强免疫力的特性。
本研究的目的是确定不同浓度的芦荟凝胶（AVG）对口腔病原菌的抗菌和抑制活性。
对20名患者进行龈下结石采集以及根尖周脓肿和牙周脓肿的抽吸，将样本转移至硫乙醇酸盐肉汤中，在变形链球菌琼脂、血琼脂中培养，并在厌氧气室中培养。通过革兰氏染色法和生化发酵试验（IMViC）进一步鉴定形成的菌落。将鉴定出的每种细菌的分离菌落分别在Muller-Hilton肉汤中培养，并在37°C下孵育24小时。通过纸片扩散法测试AVG的抗菌活性，并通过肉汤微量稀释法确定最低抑菌浓度。
各种染色和生化试验证实样本中含有（）、（）、（）和（）。AVG在100%和50%浓度时显示出抗菌特性（“t”值=7.504，p值<0.001）。在较低浓度下对细菌没有作用。在100% AVG浓度下，在（）中测量的抑菌圈为6.9mm，在（）中为6.3mm，在（）中为6.8mm，在（）中为6.6mm。还使用标准药物来比较AVG的抗菌特性。结果表明，较高浓度（100%，50%）的AVG具有与氧氟沙星（5mcg）和环丙沙星（30mcg）相当的抑菌圈。
较高浓度的AVG显示出抗菌特性，可作为口腔保健的一种有前景的辅助手段。
