Department of Medical Imaging, Jinling Hospital, Medical School of Nanjing University, 305 Zhongshan East Road, Nangjing 210002, Jiangsu, P.R. China.
Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Centre for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, Nanjing, 210023 Jiangsu, P.R. China.
Nanoscale. 2017 Jan 26;9(4):1466-1474. doi: 10.1039/c6nr07598c.
To date, clinicians still lack an effective strategy to treat triple negative breast cancer (TNBC). In this work, we design for the first time a gold nanorod embedded large-pore mesoporous organosilica (GNR@LPMO) nanoplatform for gene and photothermal cooperative therapy of TNBC. The synthesized GNR@LPMOs possess a uniform size (175 nm), high surface area (631 m g), large pore size, excellent photothermal efficiency, and good biocompatibility. Thanks to the large-pore mesoporous organosilica layer, the GNR@LPMO nanoplatforms display much higher loading capacity of siRNA compared with traditional liposome and bare gold nanorods. Thus, functional siRNA can be efficiently delivered into TNBC cells by GNR@LPMOs, causing much higher cell apoptosis through knocking down the PLK1 proteins. By combining the effective gene delivery and photothermal abilities, the GNR@LPMO nanoplatforms are further used for gene and photothermal cooperative therapy of TNBC, which induce a 15 fold higher mice tumor inhibition rate than sole therapy modality, indicating the potential clinical use of this novel nanoplatform in treating TNBC.
迄今为止，临床医生仍然缺乏治疗三阴性乳腺癌 (TNBC) 的有效策略。在这项工作中，我们首次设计了一种金纳米棒嵌入的大孔介孔有机硅 (GNR@LPMO) 纳米平台，用于 TNBC 的基因和光热协同治疗。合成的 GNR@LPMO 具有均匀的尺寸 (175nm)、高比表面积 (631m²/g)、大孔径、优异的光热效率和良好的生物相容性。由于大孔介孔有机硅层的存在，GNR@LPMO 纳米平台显示出比传统脂质体和裸露的金纳米棒更高的载 siRNA 能力。因此，功能性 siRNA 可以通过 GNR@LPMO 有效地递送到 TNBC 细胞中，通过敲低 PLK1 蛋白导致更高的细胞凋亡。通过结合有效的基因传递和光热能力，GNR@LPMO 纳米平台进一步用于 TNBC 的基因和光热协同治疗，与单一治疗方式相比，诱导小鼠肿瘤抑制率提高了 15 倍，表明这种新型纳米平台在治疗 TNBC 方面具有潜在的临床应用价值。
