Li Yong, Ai Meng-Jie, Sun Ye, Zhang Yu-Qin, Zhang Jian-Qiang
Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong Universit, Chengdu 610031, PR China.
Institute of Mediicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3144-3149. doi: 10.1099/ijsem.0.001778. Epub 2017 Aug 25.
A phosphate-solubilizing bacterium, designated CPCC 100624T, was isolated from a freshwater reservoir in south-west China. The 16S rRNA gene sequence comparison of strain CPCC 100624T with the available sequences in the GenBank database showed that the isolate was closely related to members of the genus Spirosoma. In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 100624T formed a stable phylogenetic subclade with Spirosoma soli MIMBbqt12T within the genus Spirosoma, which indicated that strain CPCC 100624T could be identified as a member of the genus Spirosoma. The strain grew at 10-40 °C (optimum 30 °C), at pH 6.5-8.0 (optimum pH 7.0-7.5) and in the presence of 0-5 % (w/v) NaCl (optimum 0-1 %). MK-7 was detected as the main menaquinone, with a minor amount of MK-7(H6) in its menaquinone system. Cells contained summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C16 : 1ω5c and iso-C15 : 0 as the major fatty acids. The polar lipids of strain CPCC 100624T contained phosphatidylethanolamine, one unidentified aminolipid, two aminophospholipids and three unidentified lipids. The genomic DNA G+C content was 49.3 mol%. On the basis of the above taxonomic data and differences in physiological characteristics from the closely related type strains, strain CPCC 100624T represents a novel species of the genus Spirosoma, for which the name Spirosoma lacussanchae sp. nov. is proposed. The type strain is CPCC 100624T (=NBRC 111852T=DSM 101771T).
从中国西南部的一个淡水水库中分离出一株解磷细菌，命名为CPCC 100624T。对菌株CPCC 100624T的16S rRNA基因序列与GenBank数据库中现有序列进行比较，结果表明该分离株与螺菌属（Spirosoma）的成员密切相关。在基于16S rRNA基因序列构建的系统发育树中，菌株CPCC 100624T与螺菌属中的土壤螺菌（Spirosoma soli）MIMBbqt12T形成了一个稳定的系统发育亚分支，这表明菌株CPCC 100624T可被鉴定为螺菌属的一个成员。该菌株在10-40℃（最适温度30℃）、pH 6.5-8.0（最适pH 7.0-7.5）以及0-5%（w/v）NaCl（最适0-1%）的条件下生长。检测到MK-7为主要甲基萘醌，其甲基萘醌系统中含有少量的MK-7(H6)。细胞中主要脂肪酸为总特征3（C16:1ω6c和/或C16:1ω7c）、C16:1ω5c和异-C15:0。菌株CPCC 100624T的极性脂类包含磷脂酰乙醇胺、一种未鉴定的氨基脂、两种氨基磷脂和三种未鉴定的脂类。基因组DNA的G+C含量为49.3mol%。基于上述分类学数据以及与密切相关模式菌株在生理特征上的差异，菌株CPCC 100624T代表了螺菌属的一个新物种，为此提出新名称湖生螺菌（Spirosoma lacussanchae）。模式菌株为CPCC 100624T（=NBRC 111852T=DSM 101771T）。
