Institute for Physical &Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Rebenring 56, 38106 Braunschweig, Germany.
Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina.
Nat Commun. 2017 Jan 11;8:13966. doi: 10.1038/ncomms13966.
Over the last decade, two fields have dominated the attention of sub-diffraction photonics research: plasmonics and fluorescence nanoscopy. Nanoscopy based on single-molecule localization offers a practical way to explore plasmonic interactions with nanometre resolution. However, this seemingly straightforward technique may retrieve false positional information. Here, we make use of the DNA origami technique to both control a nanometric separation between emitters and a gold nanoparticle, and as a platform for super-resolution imaging based on single-molecule localization. This enables a quantitative comparison between the position retrieved from single-molecule localization, the true position of the emitter and full-field simulations. We demonstrate that plasmonic coupling leads to shifted molecular localizations of up to 30 nm: a single-molecule mirage.
在过去的十年中，两个领域主导了亚衍射光子学研究的注意力：等离子体学和荧光纳米显微镜。基于单分子定位的纳米显微镜为探索等离子体相互作用提供了一种实用的方法，分辨率达到纳米级。然而，这种看似简单的技术可能会检索到错误的位置信息。在这里，我们利用 DNA 折纸技术来控制发射器和金纳米颗粒之间的纳米级分离，并将其作为基于单分子定位的超分辨率成像的平台。这使得可以在单分子定位中检索到的位置、发射器的真实位置和全场模拟之间进行定量比较。我们证明了等离子体耦合导致分子定位的偏移高达 30nm：单分子海市蜃楼。
