Deshmukh Pravin Suryakantrao, Megha Kanu, Nasare Namita, Banerjee Basu Dev, Ahmed Rafat Sultana, Abegaonkar Mahesh Pandurang, Tripathi Ashok Kumar, Mediratta Pramod Kumari
Environmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry. University College of Medical Sciences & G.T.B. Hospital (University of Delhi), Dilshad Garden, Delhi 110095, India.
Environmental Biochemistry and Molecular Biology Laboratory, Department of Biochemistry. University College of Medical Sciences & G.T.B. Hospital (University of Delhi), Dilshad Garden, Delhi 110095, India; Department of Pharmacology, University College of Medical Sciences & G.T.B. Hospital (University of Delhi), Dilshad Garden, Delhi 110095, India
Biomed Environ Sci. 2016 Dec;29(12):858-867. doi: 10.3967/bes2016.115.
The present study was designed to investigate the effects of subchronic low level microwave radiation (MWR) on cognitive function, heat shock protein 70 (HSP70) level and DNA damage in brain of Fischer rats.
Experiments were performed on male Fischer rats exposed to microwave radiation for 90 days at three different frequencies: 900, 1800, and 2450 MHz. Animals were divided into 4 groups: Group I: Sham exposed, Group II: animals exposed to microwave radiation at 900 MHz and specific absorption rate (SAR) 5.953 × 10-4 W/kg, Group III: animals exposed to 1800 MHz at SAR 5.835 × 10-4 W/kg and Group IV: animals exposed to 2450 MHz at SAR 6.672 × 10-4 W/kg. All the animals were tested for cognitive function using elevated plus maze and Morris water maze at the end of the exposure period and subsequently sacrificed to collect brain tissues. HSP70 levels were estimated by ELISA and DNA damage was assessed using alkaline comet assay.
Microwave exposure at 900-2450 MHz with SAR values as mentioned above lead to decline in cognitive function, increase in HSP70 level and DNA damage in brain.
The results of the present study suggest that low level microwave exposure at frequencies 900, 1800, and 2450 MHz may lead to hazardous effects on brain.
本研究旨在探讨亚慢性低水平微波辐射（MWR）对Fischer大鼠认知功能、热休克蛋白70（HSP70）水平及脑内DNA损伤的影响。
以雄性Fischer大鼠为实验对象，分别在900、1800和2450 MHz这三种不同频率下暴露于微波辐射90天。动物被分为4组：第一组：假暴露组；第二组：暴露于900 MHz微波辐射且比吸收率（SAR）为5.953×10⁻⁴W/kg的动物组；第三组：暴露于1800 MHz且SAR为5.835×10⁻⁴W/kg的动物组；第四组：暴露于2450 MHz且SAR为6.672×10⁻⁴W/kg的动物组。在暴露期结束时，使用高架十字迷宫和Morris水迷宫对所有动物进行认知功能测试，随后处死动物以收集脑组织。通过酶联免疫吸附测定法（ELISA）评估HSP70水平，并使用碱性彗星试验评估DNA损伤。
上述SAR值下，900 - 2450 MHz的微波暴露导致大鼠认知功能下降、脑内HSP70水平升高及DNA损伤。
本研究结果表明，900、1800和2450 MHz频率的低水平微波暴露可能对大脑产生有害影响。
