信使核糖核酸中碱基修饰的化学及结构效应
Chemical and structural effects of base modifications in messenger RNA.
作者信息
Harcourt Emily M, Kietrys Anna M, Kool Eric T
机构信息
Department of Chemistry, Wellesley College, Wellesley, Massachusetts 02481, USA.
Department of Chemistry, Stanford University, Stanford, California 94305, USA.
出版信息
Nature. 2017 Jan 18;541(7637):339-346. doi: 10.1038/nature21351.
Abstract
A growing number of nucleobase modifications in messenger RNA have been revealed through advances in detection and RNA sequencing. Although some of the biochemical pathways that involve modified bases have been identified, research into the world of RNA modification - the epitranscriptome - is still in an early phase. A variety of chemical tools are being used to characterize base modifications, and the structural effects of known base modifications on RNA pairing, thermodynamics and folding are being determined in relation to their putative biological roles.
摘要
通过检测技术和RNA测序技术的进步，信使RNA中越来越多的核碱基修饰被揭示出来。尽管一些涉及修饰碱基的生化途径已被确定，但对RNA修饰领域——表观转录组的研究仍处于早期阶段。各种各样的化学工具正被用于表征碱基修饰，并且已知碱基修饰对RNA配对、热力学和折叠的结构影响正根据其假定的生物学作用进行测定。
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