（）茎皮提取物及其馏分的抗炎和抗伤害感受活性
Anti-Inflammatory and Anti-Nociceptive Activities of Stem-Bark Extracts and Fractions of ().
作者信息
Nwidu Lucky Legbosi, Airhihen Blessing, Ahmadu Augustine
机构信息
Department of Experimental Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Port Harcourt, Port Harcourt, Rivers State, Nigeria.
Department of Pharmacology and Toxicology, Faculty Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.
出版信息
J Basic Clin Pharm. 2016 Dec;8(1):25-32. doi: 10.4103/0976-0105.195097.
BACKGROUND
In Niger Delta, ethnomedicine hydroalcoholic extract of (CL) () is used to relieve inflammatory pains.
OBJECTIVES
The purpose of this study is to evaluate the anti-inflammatory and antinociceptive effects of ethanolic stem extract (ESE) and to fractionate the ESE for the elucidation of bioactive molecules.
MATERIALS AND METHODS
The antinociceptive effects for ESE were tested against two noxious stimuli; chemical (acetic acid-induced writhing and formalin-induced pain) and thermal (hot plate) stimuli. The effects of paracetamol (130 mg/kg), indomethacin (10 mg/kg), and morphine (5 mg/kg) pretreatment were investigated. To isolate the bioactive compounds with anti-inflammatory effect, two doses (86.6 and 173.2 mg/kg) of four fractions (methanol fraction MTF, ethyl acetate fraction EAF, chloroform fraction CHF, and n-hexane fraction n-HF) obtained from fractionating ESE were utilized. Carrageenan, egg albumin, and capsaicin-induced edema of the hind paw of the rats were the models adopted. Paw volume was measured by a digital vernier caliper from 0 to 6 h after injection. This was compared to standard drugs. The results were subjected to statistical analysis.
RESULTS
The ESE decreased significantly ( < 0.001) the writhing of acetic acid-induced abdominal contractions and licking of formalin-induced pains but does not have any effects on the hot plate test. Of the four fractions obtained, the EAFs demonstrated a significant ( < 0.001) inflammatory inhibition of 98.97% and 41.91% at 86.6 and 173.2 mg/kg, respectively, compared to 65.75% inhibition demonstrated by the reference drug, acetylsalicylic acid (100 mg/kg) on the carrageenan model while 36.36% and 29.87% inhibition of inflammation at 86.6 and 173.2 mg/kg, respectively, on the egg albumin models; there was no significant effect on the capsaicin model.
CONCLUSION
The isolation of quercetin and kaemferol from CL gave credence to its anti-inflammatory and antinociceptive effects.
背景
在尼日尔三角洲，可乐果（CL）的乙醇提取物被用于缓解炎性疼痛。
目的
本研究旨在评估可乐果乙醇茎提取物（ESE）的抗炎和镇痛作用，并对ESE进行分离以阐明其生物活性分子。
材料与方法
针对两种有害刺激测试ESE的镇痛作用；化学刺激（乙酸诱导扭体和福尔马林诱导疼痛）和热刺激（热板法）。研究了对乙酰氨基酚（130mg/kg）、吲哚美辛（10mg/kg）和吗啡（5mg/kg）预处理的效果。为分离具有抗炎作用的生物活性化合物，使用了从ESE分离得到的四个馏分（甲醇馏分MTF、乙酸乙酯馏分EAF、氯仿馏分CHF和正己烷馏分n-HF）的两个剂量（86.6和173.2mg/kg）。采用角叉菜胶、蛋清和辣椒素诱导大鼠后爪水肿的模型。在注射后0至6小时用数字游标卡尺测量爪体积。将其与标准药物进行比较。对结果进行统计分析。
结果
ESE显著降低（P<0.001）乙酸诱导的腹部收缩扭体次数和福尔马林诱导疼痛的舔舐次数，但对热板试验无任何影响。在得到的四个馏分中，EAF在86.6和173.2mg/kg时分别表现出显著的（P<0.001）抗炎作用，抑制率分别为98.97%和41.91%，相比之下，参考药物乙酰水杨酸（100mg/kg）对角叉菜胶模型的抑制率为65.75%；在蛋清模型中，86.6和173.2mg/kg时的炎症抑制率分别为36.36%和29.87%；对辣椒素模型无显著影响。
结论
从可乐果中分离出槲皮素和山奈酚证实了其抗炎和镇痛作用。
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