Zhang W J, Shang X L, Peng J, Zhou M H, Sun W J
Department of Neurology, Dongying People's Hospital, Dongying, China
Department of Neurology, First Affiliated Hospital, China Medical University, Shenyang, China.
Genet Mol Res. 2017 Jan 23;16(1):gmr-16-01-gmr.16019022. doi: 10.4238/gmr16019022.
Parkinson's disease (PD) is one of the most common neurodegenerative diseases and mainly manifests with decreasing numbers of dopaminergic neurons. Rapid eye movement (REM) sleep behavior disorder (RBD) has an incidence of 15-47% in all PD patients. Prion proteins (PrPs), which are expressed in both neurons and glial cells of the brain, are believed to be correlated with abnormal neurological functions, although their role in PD-related sleeping disorders remains unclear. We therefore investigated the expressional profiles of PrP in PD patients with RBD. Quantitative real-time polymerase chain reaction and western blotting were used to detect the mRNA and protein levels of PrP, respectively, in the cerebrospinal fluid (CSF) of PD patients with RBD, PD patients without sleeping disorder, and healthy people (N = 23 each). We investigated the correlation between the CSF PrP level and sleeping behavior in PD patients. Patients with PD complicated with RBD had significantly elevated CSF PrP expression levels (both mRNA and protein) compared with either PD patients without sleeping disorder or healthy individuals (P < 0.05 in both cases). There is elevated expression of PrP in the CSF of PD patients with RBD. This may benefit the diagnosis of PD-related RBD.
帕金森病（PD）是最常见的神经退行性疾病之一，主要表现为多巴胺能神经元数量减少。快速眼动（REM）睡眠行为障碍（RBD）在所有PD患者中的发病率为15%至47%。朊病毒蛋白（PrP）在大脑的神经元和神经胶质细胞中均有表达，尽管其在PD相关睡眠障碍中的作用尚不清楚，但人们认为它与神经功能异常有关。因此，我们研究了患有RBD的PD患者中PrP的表达谱。采用定量实时聚合酶链反应和蛋白质印迹法分别检测患有RBD的PD患者、无睡眠障碍的PD患者和健康人（每组N = 23）脑脊液（CSF）中PrP的mRNA和蛋白质水平。我们研究了PD患者脑脊液PrP水平与睡眠行为之间的相关性。与无睡眠障碍的PD患者或健康个体相比，合并RBD的PD患者脑脊液PrP表达水平（mRNA和蛋白质）均显著升高（两种情况均P < 0.05）。患有RBD的PD患者脑脊液中PrP表达升高。这可能有助于PD相关RBD的诊断。
