Ethnomedicinal Seed Extracts: An Impending Preparation against Multidrug Resistant Bacterial Pathogens.

BACKGROUND

The present study was undertaken to analyze the phytochemical content and biological activity of seeds traditionally used in Charsadda, Pakistan against multidrug resistant (MDR) bacterial pathogens.

OBJECTIVES

This study explored the qualitative and quantitative antibacterial potential of . Further qualitative analysis of phytochemical content was performed.

METHODS

seed extracts were prepared in aqueous, chloroform, ethanol, and hexane separately.

RESULTS

All the extracts of seeds were screened for antibacterial activity and phytochemical content. seed extract showed considerable activity against MDR pathogenic bacteria. In the well diffusion method, aqueous extracts showed a higher zone of inhibition against (16 mm ± 0.7 mm) and (13 mm ± 0.5 mm), whereas chloroform, ethanol, and hexane extracts showed activity against (11 mm ± 0.3 mm, 12 mm ± 0.5 mm, and 11 mm ± 0 mm, respectively) as compared to Imipenem, a broad spectrum antibiotic. Minimum inhibitory concentration and minimum bactericidal concentration values for aqueous and ethanol extracts indicate that they were more effective against MDR bacteria. Phytochemical analysis revealed that aqueous and ethanol extracts were rich in alkaloids, carbohydrates, gallotannins, and triterpenoids, whereas chloroform and hexane extracts were more concentrated with phenolics, pseudotannins, saponins, and tannins. seed extract demonstrated potential activity against MDR human pathogenic bacteria.

CONCLUSIONS

The undertaken study has for the first time reported the effects of seed extracts against MDR bacterial pathogens. Findings of the current study will be helpful for further elucidation of bioactive molecules for therapeutic use against MDR bacterial pathogens.