Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, Department of Preventive Medicine, Third Military Medical University , Chongqing, 400038, China.
ACS Appl Mater Interfaces. 2017 Mar 1;9(8):6839-6848. doi: 10.1021/acsami.6b15442. Epub 2017 Feb 13.
Although ceria-based nanostructures have emerged as fascinating materials with diverse biological activities, developing a facile, rapid, and biocompatible method of their preparation remains a major challenge. Herein we describe bovine serum albumin (BSA) protein-directed synthesis of ceria-based nanostructures, including ceria nanoclusters (CNLs), nanoparticles (CNPs), and nanochains (CNHs). Their preparation is simple, one-pot, and performed in a mild reaction condition with a "green" synthetic approach. Most importantly, these three kinds of ceria-based nanostructures can be synthesized in a shape and size controllable manner by tuning the reaction time, temperature, and molar ratio. The formation mechanism shows that growth of these ceria nanostructures is mediated by Ce/Ce switchable redox system, reducible disulfide bonds, and unique spatial structures in albumin proteins. More importantly, these albumin-based ceria nanostructures exhibit high superoxide dismutase (SOD) mimetic activity and good biocompatibility, providing a promising prospect in biomedical application.
尽管基于氧化铈的纳米结构作为具有多种生物活性的迷人材料已经出现，但开发一种简便、快速和生物相容的制备方法仍然是一个主要挑战。在此，我们描述了牛血清白蛋白（BSA）指导的基于氧化铈的纳米结构的合成，包括氧化铈纳米团簇（CNLs）、纳米颗粒（CNPs）和纳米链（CNHs）。它们的制备简单、一锅法，在温和的反应条件下采用“绿色”合成方法进行。最重要的是，通过调节反应时间、温度和摩尔比，可以以可控的方式合成这三种基于氧化铈的纳米结构。形成机制表明，这些氧化铈纳米结构的生长是由 Ce/Ce 可切换氧化还原体系、可还原的二硫键和白蛋白蛋白质中的独特空间结构介导的。更重要的是，这些基于白蛋白的氧化铈纳米结构表现出高的超氧化物歧化酶（SOD）模拟活性和良好的生物相容性，为生物医学应用提供了有前途的前景。
