法属圭亚那循环的皮肤前鞭毛体分离株对一组药物的体外敏感性
In Vitro Sensitivity of Cutaneous Promastigote Isolates Circulating in French Guiana to a Set of Drugs.
作者信息
Ginouvès Marine, Simon Stéphane, Nacher Mathieu, Demar Magalie, Carme Bernard, Couppié Pierre, Prévot Ghislaine
机构信息
Laboratoire Associé, Centre National de Référence Leishmania, Laboratoire Hospitalo-Universitaire de Parasitologie et Mycologie, General Hospital, Cayenne, French Guiana.
Laboratoire des Ecosystèmes Amazoniens et Pathologie Tropicale (EPaT), Université de Guyane, Labex CEBA, DFR Santé, Cayenne, French Guiana.
出版信息
Am J Trop Med Hyg. 2017 May;96(5):1143-1150. doi: 10.4269/ajtmh.16-0373. Epub 2017 Feb 6.
Abstract
AbstractAnti-leishmaniasis drug resistance is a common problem worldwide. The aim of this study was to inventory the general in vitro level of sensitivity of isolates circulating in French Guiana and to highlight potential in vitro pentamidine-resistant isolates. This sensitivity study was conducted on 36 patient-promastigote isolates for seven drugs (amphotericin B, azithromycin, fluconazole, meglumine antimoniate, miltefosine, paromomycin, and pentamidine) using the Cell Counting Kit-8 viability test. The IC values obtained were heterogeneous. One isolate exhibited high IC values for almost all drugs tested. Pentamidine, which is the first-line treatment in French Guiana, showed efficacy at very low doses (mean of 0.0038 μg/mL). The concordance of the in vitro pentamidine results with the patients' clinical outcomes was 94% ( = 0.82).
摘要
摘要
抗利什曼病药物耐药性是全球普遍存在的问题。本研究的目的是清查法属圭亚那流行的分离株的总体体外敏感水平，并找出潜在的体外耐喷他脒分离株。使用细胞计数试剂盒-8活力试验，对36株患者前鞭毛体分离株进行了七种药物（两性霉素B、阿奇霉素、氟康唑、葡甲胺锑酸盐、米替福新、巴龙霉素和喷他脒）的敏感性研究。获得的IC值具有异质性。一株分离株对几乎所有测试药物均表现出高IC值。喷他脒是法属圭亚那的一线治疗药物，在极低剂量（平均0.0038μg/mL）时显示出疗效。体外喷他脒结果与患者临床结局的一致性为94%（κ=0.82）。
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