Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai 200092, China; Center for Human Tissues and Organs Degeneration, Shenzhen Institute of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, China.
Institute of Bioengineering and Information Technology Materials, Tongji University, Shanghai 200092, China.
Mater Sci Eng C Mater Biol Appl. 2017 Apr 1;73:585-595. doi: 10.1016/j.msec.2016.12.101. Epub 2016 Dec 22.
There is a need for synthetic biomaterials to heal bone defects using minimal invasive surgery. In the present study, an injectable cement composed of bioactive borate glass particles and a chitosan bonding solution was developed and evaluated for its capacity to heal bone defects in a rabbit femoral condyle model. The injectability and setting time of the cement in vitro decreased but the compressive strength increased (8±2MPa to 31±2MPa) as the ratio of glass particles to chitosan solution increased (from 1.0gml to 2.5gml). Upon immersing the cement in phosphate-buffered saline, the glass particles reacted and converted to hydroxyapatite, imparting bioactivity to the cement. Osteoblastic MC3T3-E1 cells showed enhanced proliferation and alkaline phosphatase activity when incubated in media containing the soluble ionic product of the cement. The bioactive glass cement showed a better capacity to stimulate bone formation in rabbit femoral condyle defects at 12weeks postimplantation when compared to a commercial calcium sulfate cement. The injectable bioactive borate glass cement developed in this study could provide a promising biomaterial to heal bone defects by minimal invasive surgery.
需要使用微创外科技术来治疗骨缺损的合成生物材料。在本研究中，开发了一种由生物活性硼酸盐玻璃颗粒和壳聚糖粘结溶液组成的可注射水泥，并对其在兔股骨髁模型中修复骨缺损的能力进行了评估。随着玻璃颗粒与壳聚糖溶液的比例（从 1.0g/ml 增加到 2.5g/ml）增加，水泥的可注射性和体外凝固时间降低，但抗压强度增加（从 8±2MPa 增加到 31±2MPa）。当水泥浸泡在磷酸盐缓冲盐溶液中时，玻璃颗粒发生反应并转化为羟基磷灰石，使水泥具有生物活性。成骨细胞 MC3T3-E1 细胞在含有水泥可溶性离子产物的培养基中孵育时，增殖和碱性磷酸酶活性增强。与商业硫酸钙水泥相比，在植入后 12 周时，可注射生物活性硼酸盐玻璃水泥在兔股骨髁缺损中显示出更好的刺激骨形成能力。本研究开发的可注射生物活性硼酸盐玻璃水泥可能为微创外科治疗骨缺损提供一种有前途的生物材料。
