Institute for Nanobiotechnology, John Hopkins University , 3400 North Charles Street, Baltimore, Maryland 21218, United States.
Department of Mechanical Engineering, John Hopkins University , 3400 North Charles Street, Baltimore, Maryland 21218, United States.
Anal Chem. 2016 Dec 20;88(24):12241-12247. doi: 10.1021/acs.analchem.6b03391. Epub 2016 Nov 29.
The components of sweat provide an array of potential biomarkers for health and disease. Sweat chloride is of interest as a biomarker for cystic fibrosis, electrolyte metabolism disorders, electrolyte balance, and electrolyte loss during exercise. Developing wearable sensors for biomarkers in sweat is a major technological challenge. Potentiometric sensors provide a relatively simple technology for on-body sweat chloride measurement, however, equilibration between reference and test solutions has limited the time over which accurate measurements can be made. Here, we report on a wearable potentiometric chloride sweat sensor. We performed parametric studies to show how the salt bridge geometry determines equilibration between the reference and test solutions. From these results, we show a sweat chloride sensor can be designed to provide accurate measurements over extended times. We then performed on-body tests on healthy subjects while exercising to establish the feasibility of using this technology as a wearable device.
汗液成分提供了一系列潜在的健康和疾病生物标志物。汗液氯化物作为囊性纤维化、电解质代谢紊乱、电解质平衡以及运动时电解质丢失的生物标志物备受关注。开发用于汗液生物标志物的可穿戴传感器是一项重大技术挑战。 电势传感器为体汗氯化物测量提供了相对简单的技术，但是参考溶液和测试溶液之间的平衡限制了可以进行准确测量的时间。在这里，我们报告了一种可穿戴的电势氯化物汗液传感器。我们进行了参数研究，以展示盐桥几何形状如何决定参考溶液和测试溶液之间的平衡。根据这些结果，我们展示了可以设计一种汗液氯化物传感器，以在较长时间内提供准确的测量结果。然后，我们在健康受试者运动时进行了体上测试，以证明将该技术用作可穿戴设备的可行性。
