State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, China.
Center for Bioelectronics and Biosensors, Biodesign Institute, Arizona State University , Tempe, Arizona 85287, United States.
Anal Chem. 2017 Mar 7;89(5):2704-2707. doi: 10.1021/acs.analchem.6b05049. Epub 2017 Feb 21.
Surface plasmon resonance microscopy (SPRM) is a powerful platform for biomedical imaging and molecular binding kinetics analysis. However, the spatial resolution of SPRM along the plasmon propagation direction (longitudinal) is determined by the decaying length of the plasmonic wave, which can be as large as tens of microns. Different methods have been proposed to improve the spatial resolution, but each at the expense of decreased sensitivity or temporal resolution. Here we present a method to achieve high spatial resolution SPRM based on deconvolution of complex field. The method does not require additional optical setup and improves the spatial resolution in the longitudinal direction. We applied the method to image nanoparticles and achieved close-to-diffraction limit resolution in both longitudinal and transverse directions.
表面等离子体共振显微镜 (SPRM) 是一种用于生物医学成像和分子结合动力学分析的强大平台。然而，SPRM 沿等离子体传播方向（纵向）的空间分辨率取决于等离子体波的衰减长度，该长度可达数十微米。已经提出了不同的方法来提高空间分辨率，但每种方法都以降低灵敏度或时间分辨率为代价。在这里，我们提出了一种基于复场反卷积的高空间分辨率 SPRM 方法。该方法不需要额外的光学设置，可以提高纵向的空间分辨率。我们将该方法应用于纳米粒子成像，在纵向和横向都实现了接近衍射极限的分辨率。
