Kanth M Rajini, Prakash A Ravi, Sreenath G, Reddy Vikram Simha, Huldah S
Research Scholar, Pacific University , Udaipur, Rajasthan, India .
Professor and Head, Department of Oral Pathology, G. Pulla Reddy Dental College and Hospital , Kurnool, Andhra Pradesh, India .
J Clin Diagn Res. 2016 Dec;10(12):ZM01-ZM03. doi: 10.7860/JCDR/2016/19772.9025. Epub 2016 Dec 1.
Dental caries and periodontal diseases are the most common oral diseases seen globally, both in developed and developing countries. Oral microorganisms that is gram positive and gram negative bacteria are known to be involved in causation of these diseases. Nowadays commercially available dentrifices and mouth rinses are known to contain ingredients that can alter the oral microbial flora and have undesirable side effects such as vomiting, diarrhoea, disarrangement of oral, intestinal flora and tooth staining. Naturally available plant products are known to be less harmful with fewer side effects and also economical for the patient.
The aim of this study was to determine the antimicrobial properties of 10 naturally available plant products against oral microorganisms causing caries and to check the efficacy of these products in-vitro and to use these in mouth washes and dentrifices.
Sample of caries material was scrapped out from the extracted teeth and transferred to liquid broth, streaked over the agar media to allow for the growth of microorganisms. Plant products like clove oil, neem, ginger-garlic paste, tea tree oil, ginger, garlic, cinnamon oil, green tea, eucalyptus oil and turmeric were used. Antimicrobial efficacy of these products, was estimated by measuring zones of inhibition in the nutrient agar media.
Clove oil was the most effective of all products against microorganisms causing caries with zone of inhibition - 30mm followed by ginger-garlic paste - 25mm, Neem - 15mm, tea tree oil - 15mm.
Based on the above results, it can be inferred that these natural products have the maximum efficacy against microorganisms and can be recommended in dentifrices, mouth rinses, topical gels, etc.
龋齿和牙周疾病是全球发达国家和发展中国家最常见的口腔疾病。已知革兰氏阳性菌和革兰氏阴性菌等口腔微生物与这些疾病的病因有关。如今，市售的牙膏和漱口水含有可能改变口腔微生物群的成分，并具有不良副作用，如呕吐、腹泻、口腔和肠道菌群紊乱以及牙齿染色。天然植物产品已知危害较小，副作用较少，对患者来说也经济实惠。
本研究的目的是确定10种天然植物产品对导致龋齿的口腔微生物的抗菌特性，检查这些产品的体外疗效，并将其用于漱口水和牙膏中。
从拔除的牙齿上刮下龋坏物质样本，转移到液体肉汤中，在琼脂培养基上划线，以促进微生物生长。使用了丁香油、印楝、姜蒜糊、茶树油、生姜、大蒜、肉桂油、绿茶、桉叶油和姜黄等植物产品。通过测量营养琼脂培养基中的抑菌圈来评估这些产品的抗菌效果。
丁香油是所有产品中对导致龋齿的微生物最有效的，抑菌圈为30毫米，其次是姜蒜糊——25毫米，印楝——15毫米，茶树油——15毫米。
基于上述结果，可以推断这些天然产品对微生物具有最大疗效，可推荐用于牙膏、漱口水、局部凝胶等。
