Park Seongjun, Guo Yuanyuan, Jia Xiaoting, Choe Han Kyoung, Grena Benjamin, Kang Jeewoo, Park Jiyeon, Lu Chi, Canales Andres, Chen Ritchie, Yim Yeong Shin, Choi Gloria B, Fink Yoel, Anikeeva Polina
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Nat Neurosci. 2017 Apr;20(4):612-619. doi: 10.1038/nn.4510. Epub 2017 Feb 20.
Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities in a single biocompatible platform remains a challenge. We developed a device composed of an optical waveguide, six electrodes and two microfluidic channels produced via fiber drawing. Our probes facilitated injections of viral vectors carrying opsin genes while providing collocated neural recording and optical stimulation. The miniature (<200 μm) footprint and modest weight (<0.5 g) of these probes allowed for multiple implantations into the mouse brain, which enabled opto-electrophysiological investigation of projections from the basolateral amygdala to the medial prefrontal cortex and ventral hippocampus during behavioral experiments. Fabricated solely from polymers and polymer composites, these flexible probes minimized tissue response to achieve chronic multimodal interrogation of brain circuits with high fidelity.
神经通路的光遗传学研究依赖于将光敏视蛋白递送至组织中，随后对感兴趣区域进行光学照射和电记录。尽管近年来多功能神经探针有所发展，但将这些模式集成到单个生物相容性平台中仍然是一项挑战。我们开发了一种由光波导、六个电极和两个通过拉拔光纤制造的微流体通道组成的设备。我们的探针有助于注射携带视蛋白基因的病毒载体，同时提供并置的神经记录和光学刺激。这些探针的微型（<200μm）尺寸和适中的重量（<0.5g）允许在小鼠大脑中进行多次植入，从而能够在行为实验期间对从基底外侧杏仁核到内侧前额叶皮质和腹侧海马体的投射进行光电生理研究。这些柔性探针仅由聚合物和聚合物复合材料制成，将组织反应降至最低，以实现对脑回路的长期多模态高保真研究。
