Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University, Hangzhou Zhejiang, 310003, China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou Zhejiang, 310003, China.
Sci Rep. 2017 Jul 3;7(1):4491. doi: 10.1038/s41598-017-04873-5.
The nanosecond pulsed electric field (nsPEF) is investigated as an alternative plan for benign hepatic hydatid cyst. Altogether 72 C57B6 mice were included. Normal group (n = 12) had no parasite injection and the other 60 mice were used to induce hydatid cyst in liver by injecting protoscolices in portal vein. The liver hydatid cysts were exposed to nsPEF with different doses and then follow up. The standard surgery was performed as positive control. The hydatid cyst growth was monitored by ultrasound; the morphology was checked by gross anatomy and pathology was tested by H&E stain. In nsPEF-treated groups no hepatic failure nor bleeding were observed. As a comparison, in the surgery group, high post-treatment complications occurred (50%). Significant parasite growth inhibition was seen in high nsPEF dose group as compared with control group (P < 0.05). Pathological analysis confirmed destruction of hydatid cyst with sharp demarcation defined by the electrodes. Laboratory analysis showed nsPEF stimulated a time-dependent infection and recoverable liver function. The traumatic reactions defined by white blood count was significant lower than surgery groups (P < 0.05).Preliminary studies demonstrate nsPEF ablation can be applied on hepatic hydatid by inhibiting parasite growth, destructing the cyst and stimulating infections.
纳秒脉冲电场（nsPEF）被研究作为治疗肝包虫病的一种替代方案。共纳入 72 只 C57B6 小鼠。正常组（n=12）未注射寄生虫，其余 60 只小鼠通过门静脉注射原头蚴诱导肝包虫囊肿。将肝包虫囊肿暴露于不同剂量的 nsPEF 后进行随访。标准手术作为阳性对照。通过超声监测包虫囊肿的生长情况；大体解剖检查形态，H&E 染色检查病理学。在 nsPEF 治疗组中未观察到肝衰竭或出血。相比之下，在手术组中，治疗后出现了较高的并发症发生率（50%）。与对照组相比，高剂量 nsPEF 组的寄生虫生长抑制明显（P<0.05）。病理分析证实了电极定义的包虫囊肿的破坏，具有明显的界限。实验室分析表明，nsPEF 刺激了时间依赖性感染和可恢复的肝功能。白细胞计数定义的创伤反应明显低于手术组（P<0.05）。初步研究表明，nsPEF 消融可以通过抑制寄生虫生长、破坏囊肿和刺激感染来应用于肝包虫病。
