Allier C, Morel S, Vincent R, Ghenim L, Navarro F, Menneteau M, Bordy T, Hervé L, Cioni O, Gidrol X, Usson Y, Dinten J-M
Universite Grenoble Alpes, Grenoble, F-38000, France.
CEA LETI MlNATEC Campus, Grenoble, F-38054, France.
Cytometry A. 2017 May;91(5):433-442. doi: 10.1002/cyto.a.23079. Epub 2017 Feb 27.
They present results for lens-free microscopy for the imaging of dense cell culture. With this aim, they use a multiwavelength LED illumination with well separated wavelengths, together with the implementation of an appropriate holographic reconstruction algorithm. This allows for a fast and efficient reconstruction of the phase image of densely packed cells (up to 700 cells/mm ) over a large field of view of 29.4 mm . Combined with the compactness of the system which fits altogether inside an incubator, lens-free microscopy becomes a unique tool to monitor cell cultures over several days. The high contrast phase shift images provide robust cell segmentation and tracking, and enable high throughput monitoring of individual cell dimensions, dry mass, and motility. They tested the multiwavelength lens-free video-microscope over a broad range of cell lines, including mesenchymal, endothelial, and epithelial cells. © 2017 International Society for Advancement of Cytometry.
他们展示了用于密集细胞培养成像的无透镜显微镜的结果。为此，他们使用了波长分离良好的多波长LED照明，并实施了适当的全息重建算法。这使得能够在29.4平方毫米的大视野范围内快速有效地重建密集堆积细胞（高达700个细胞/平方毫米）的相位图像。结合该系统可完全安装在培养箱内的紧凑性，无透镜显微镜成为了一种在数天内监测细胞培养的独特工具。高对比度的相移图像提供了强大的细胞分割和跟踪功能，并能够对单个细胞的尺寸、干质量和运动性进行高通量监测。他们在广泛的细胞系中测试了多波长无透镜视频显微镜，包括间充质细胞、内皮细胞和上皮细胞。© 2017国际细胞计量学促进协会。
