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Multispectral total-variation reconstruction applied to lens-free microscopy.应用于无透镜显微镜的多光谱全变差重建。
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Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture.用于贴壁细胞培养动态和定量分析的无透镜视频显微镜技术。
J Vis Exp. 2018 Feb 23(132):56580. doi: 10.3791/56580.
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An objective comparison of cell-tracking algorithms.细胞追踪算法的客观比较。
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High accuracy label-free classification of single-cell kinetic states from holographic cytometry of human melanoma cells.高准确度的无标记单细胞动力学状态分类，基于人类黑色素瘤细胞的全息细胞术。
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Imaging of dense cell cultures by multiwavelength lens-free video microscopy.通过多波长无透镜视频显微镜对密集细胞培养物进行成像。
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6
Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion.使用四波横向剪切干涉术的定量相位成像进行活细胞干质量测量：精度与灵敏度探讨
J Biomed Opt. 2015;20(12):126009. doi: 10.1117/1.JBO.20.12.126009.
7
Optical detection and measurement of living cell morphometric features with single-shot quantitative phase microscopy.利用单次拍摄定量相显微镜对活细胞形态计量特征进行光学检测和测量。
J Biomed Opt. 2012 Jul;17(7):076004. doi: 10.1117/1.JBO.17.7.076004.
8
Rapid, massively parallel single-cell drug response measurements via live cell interferometry.通过活细胞干涉测量术实现快速、大规模平行的单细胞药物反应测量。
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Optical measurement of cycle-dependent cell growth.细胞周期依赖性生长的光学测量
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Measurement of adherent cell mass and growth.贴壁细胞质量和生长的测量。
Proc Natl Acad Sci U S A. 2010 Nov 30;107(48):20691-6. doi: 10.1073/pnas.1011365107. Epub 2010 Nov 10.

贴壁哺乳动物细胞的定量相成像：一项比较研究。

Quantitative phase imaging of adherent mammalian cells: a comparative study.

作者信息

Allier C, Hervé L, Mandula O, Blandin P, Usson Y, Savatier J, Monneret S, Morales S

机构信息

Univ. Grenoble Alpes, CEA, LETI, DTBS-LSIV, F-38000 Grenoble, France.

TIMC-IMAG, Uni. Grenoble Alpes, CNRS UMR 5525, France.

出版信息

Biomed Opt Express. 2019 May 14;10(6):2768-2783. doi: 10.1364/BOE.10.002768. eCollection 2019 Jun 1.

DOI:10.1364/BOE.10.002768
PMID:31259050
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6583341/
Abstract

The Quantitative phase imaging methods have several advantages when it comes to monitoring cultures of adherent mammalian cells. Because of low photo-toxicity and no need for staining, we can follow cells in a minimally invasive way over a long period of time. The ability to measure the optical path difference in a quantitative manner allows the measurement of the cell dry mass, an important metric for studying the growth kinetics of mammalian cells. Here we present and compare cell measurements obtained with three different techniques: digital holographic microscopy, lens-free microscopy and quadriwave lateral sheering interferometry. We report a linear relationship between optical volume density values measured with these different techniques and estimate the precisions of this measurement for the different individual instruments.

摘要

在监测贴壁哺乳动物细胞培养时，定量相成像方法具有多个优点。由于光毒性低且无需染色，我们能够以微创方式长时间跟踪细胞。以定量方式测量光程差的能力使得能够测量细胞干质量，这是研究哺乳动物细胞生长动力学的一个重要指标。在此，我们展示并比较了用三种不同技术获得的细胞测量结果：数字全息显微镜、无透镜显微镜和四波横向剪切干涉测量法。我们报告了用这些不同技术测量的光学体积密度值之间的线性关系，并估计了不同单个仪器该测量的精度。