Rock D L, Beam S L, Mayfield J E
Department of Veterinary Science, North Dakota State University, Fargo 58105.
J Virol. 1987 Dec;61(12):3827-31. doi: 10.1128/JVI.61.12.3827-3831.1987.
Here we have used the bovine herpesvirus type 1 (BHV-1) rabbit model together with in situ nucleic acid hybridization to identify and map viral RNA present in latently infected neurons. Radioactively labeled cloned HindIII fragments representing most of the BHV-1 genome (Cooper strain) were individually hybridized to sections of trigeminal ganglia taken from rabbits during acute and latent stages of infection. Whereas all viral genomic fragments hybridized to lytically infected tissue culture cells and to acutely infected ganglia, only HindIII fragment D (map units 0.734 to 0.842) hybridized to latently infected ganglionic neurons. Additional in situ hybridization experiments using subcloned fragments of HindIII-D further mapped the latency-related viral RNA to a 1.9-kilobase region (map units 0.734 to 0.748) of the viral genome. These results indicate that BHV-1 gene transcription is restricted during the latent phase of infection; further, they suggest that specific viral transcription may be involved in establishment or maintenance of latent BHV-1 infection.
在此，我们使用了1型牛疱疹病毒（BHV-1）兔模型，并结合原位核酸杂交技术，来鉴定和定位潜伏感染神经元中存在的病毒RNA。代表BHV-1基因组大部分区域（库珀毒株）的放射性标记克隆HindIII片段，分别与取自感染急性期和潜伏期兔子的三叉神经节切片进行杂交。虽然所有病毒基因组片段都能与裂解感染的组织培养细胞以及急性感染的神经节杂交，但只有HindIII片段D（图谱单位0.734至0.842）能与潜伏感染的神经节神经元杂交。使用HindIII-D亚克隆片段进行的额外原位杂交实验，进一步将潜伏相关病毒RNA定位到病毒基因组的一个1.9千碱基区域（图谱单位0.734至0.748）。这些结果表明，BHV-1基因转录在感染潜伏期受到限制；此外，它们还表明特定的病毒转录可能参与了潜伏性BHV-1感染的建立或维持。
